What is assay of creatinine?
A creatinine test is a measure of how well your kidneys are performing their job of filtering waste from your blood. Creatinine is a chemical compound left over from energy-producing processes in your muscles. Healthy kidneys filter creatinine out of the blood.
Why is serum creatinine test done?
Doctors use a serum creatinine test to measure kidney function. Serum creatinine is a blood test done to determine the amount of creatinine present in the blood. Doctors also use a creatinine urine test to determine the level of kidney function of a person.
What is serum creatinine determination?
Creatinine in serum or urine is determined by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm.
What is the difference between creatinine and serum creatinine?
Serum Creatinine (sCr) A serum creatinine test measures the amount of creatinine in your blood. Creatinine is a waste product that is produced from the normal wear and tear on muscles in your body. Waste products like creatinine are filtered out of the blood by healthy kidneys and leave your body in your urine.
What is a bad creatinine level?
What are considered high creatinine levels? A person with only one kidney may have a normal level of about 1.8 or 1.9. Creatinine levels that reach 2.0 or more in babies and 5.0 or more in adults may indicate severe kidney impairment.
What are symptoms of high creatinine?
What are the symptoms of high creatinine levels?
- Nausea.
- Chest Pain.
- Muscle Cramps.
- Vomiting.
- Fatigue.
- Changes in urination frequency and appearance.
- High blood pressure.
- Swelling or fluid retention.
What if serum creatinine is high?
Elevated creatinine level signifies impaired kidney function or kidney disease. As the kidneys become impaired for any reason, the creatinine level in the blood will rise due to poor clearance of creatinine by the kidneys. Abnormally high levels of creatinine thus warn of possible malfunction or failure of the kidneys.
What is normal creatinine for age?
Here are the normal values by age: 0.9 to 1.3 mg/dL for adult males. 0.6 to 1.1 mg/dL for adult females. 0.5 to 1.0 mg/dL for children ages 3 to 18 years.
Can drinking water lower your creatinine levels?
Drinking more water could lower the serum creatinine level, but does not change kidney function. Forcing excessive water intake is not a good idea.
What food should be avoided if creatinine is high?
If you’re worried about creatinine levels, avoid high-protein foods, like: Red meat. Dairy products. Eggs.
How does bioassay systems creatinine assay kit work?
BioAssay Systems creatinine assay kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample.
How is creatinine concentration measured in the Jaffe assay?
The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw sample.
How does the enzychrom creatine kinase assay kit work?
BioAssay Systems EnzyChrom™ Creatine Kinase Assay Kit is based on enzyme coupled reactions in which creatine phosphate and ADP is converted to creatine and ATP by CK, the generated ATP is used to phosphorylate glucose by hexokinase to generate glucose-6-phosphate, which is then oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase.
Is there a 96 well plate assay for creatine kinase?
Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal muscle, smooth muscle and the brain.