What does a kinase assay measure?
A kinase assay works by simply measuring the activity of the kinase. A kinase is an enzyme that aids in the transfer of a phosphate from ATP to another specific molecule. They regulate many activities in the human body. The measurement is used to test potential drugs to see how they affect kinase activity.
Is glycerol kinase present in muscles?
Glycerol kinase is absent from muscle, which precludes the use of glycerol for gluconeogenesis in that tissue (Newsholme and Taylor, 1969).
How is glycerol formed in the body?
Glycerol is synthesized from glucose via the glycolytic pathway through reduction of dihydroxyacetone-P by glycerol dehydrogenase, yielding sn-glycerol-3P8.
How do phosphatases work?
A phosphatase is an enzyme that removes a phosphate group from a protein. Together, these two families of enzymes act to modulate the activities of the proteins in a cell, often in response to external stimuli.
Is glycerol kinase present in adipose tissue?
Since glycerol kinase activity is absent in white adipose tissue, glycerol becomes a waste product of lipolysis, and is converted to glucose in the liver, and to a lesser extent in the kidney.
What is glycerol structure?
C3H8O3
Glycerol/Formula
Which is the first method for glycerol kinase?
The first method is based on the glycerokinase/glycerolphosphate oxidase/horseradish peroxidase-coupled assay. Briefly, the assay is composed of a three-step enzymatic reaction: 1. ATP (from a measured sample) + glycerol + glycerokinase → glycerol-3-phosphate + ADP 2.
How are glycerol reagents used in bioassay systems?
Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. BioAssay Systems glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step.
How is the color intensity of a glycerol assay determined?
BioAssay Systems glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glycerol concentration in the sample.
Where is glycerol kinase absent in the liver?
Since glycerol kinase activity is absent in white adipose tissue, glycerol becomes a waste product of lipolysis, and is converted to glucose in the liver, and to a lesser extent in the kidney.