What is MDA MB 435?
MDA-MB-435, a member of the NCI-DTP panel of 60 human tumor cell lines, has been used for decades as a model of metastatic human breast cancer. This cell line was derived at M.D. Anderson in 1976 from a pleural effusion from a 31-year old woman with a history of breast cancer (Cailleau R, Olive M, Cruciger QV.
What type of breast cancer is MDA MB 231?
MDA-MB-231 is a highly aggressive, invasive and poorly differentiated triple-negative breast cancer (TNBC) cell line as it lacks oestrogen receptor (ER) and progesterone receptor (PR) expression, as well as HER2 (human epidermal growth factor receptor 2) amplification2,3.
What is MDA MB 468 cell line?
MDA-MB-468 is a cell line that was isolated from a 51-year-old female human in 1977, and is commonly used in breast cancer research.
What is the doubling time for MDA-MB-231?
Hi Nairrita, the doubling time of MDA-MB-231 cells is about 38h according to ATCC. I usually start with the density of 7,500 cells/cm2 in 96-well plate for 3 days MTS assay.
What is the difference between MCF 7 and MDA-MB-231?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What are t47d cells?
T-47D is a human breast cancer cell line commonly used in biomedical research involving the hormonal expression of cancer cells.
Is MCF-7 triple negative?
What is ovcar3?
NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
Is the MDA MB-435 a breast cancer cell line?
MDA-MB-435, and its derivation MDA-N, are Melanoma cell lines, not breast cancer cell lines. MDA-MB-435, a member of the NCI-DTP panel of 60 human tumor cell lines, has been used for decades as a model of metastatic human breast cancer. This cell line was derived at M.D. Anderson in 1976 from a pleural effusion from a 31-year old woman…
Are there any breast cancer cells derived from M14 melanoma cells?
All currently available stocks of MDA-MB-435 cells are derived from the M14 melanoma cell line and can no longer be considered a model of breast cancer. These cells are still a valuable system for the study of cancer metastasis and the extensive literature using these cells since 1982 represent a va …
Are there Breast specific genes in melanoma cells?
Breast-specific genes were not detectably expressed in MDA-MB-435 or in the melanoma lines, but were detected in most of the breast tumor cell lines as well as normal breast. However, melanocyte-specific genes were expressed in MDA-MB-435, as well as in most of the melanoma lines, but were not detectable in the other breast tumor cell lines.
Are there any undifferentiated breast cancer cell lines?
One is that the breast cancer cells are undifferentiated and express markers of melanocyte differentiation but are in fact breast cancer. Recent work has shown that primary breast tumors can express melanocyte related genes and found similar melanocyte related gene expression in the MDA-MB-435 cell line [ 26 ].