What will a gene expression profile tell you?

What will a gene expression profile tell you?

A laboratory method that identifies all of the genes in a cell or tissue that are making messenger RNA. A gene expression profile may be used to find and diagnose a disease or condition or to see how well the body responds to treatment.

What are the four levels of gene expression?

Control of gene expression in eukaryotic cells occurs at epigenetic, transcriptional, post-transcriptional, translational, and post-translational levels.

What are the three types of gene expression?

Contents

• 1.1 Transcription.
• 1.2 mRNA processing.
• 1.3 Non-coding RNA maturation.
• 1.4 RNA export.
• 1.5 Translation.
• 1.6 Folding.
• 1.7 Translocation.
• 1.8 Protein transport.

What is an NX value?

The consensus normalized expression (“NX”) value is calculated as the maximum NX value for each gene in the three data sources. For tissues with multiple sub-tissues (brain regions, blood cells, lymphoid tissues and intestine) the maximum of all sub-tissues is used for the tissue type.

What does it mean if a gene is expressed?

Gene expression is the process by which the instructions in our DNA are converted into a functional product, such as a protein. It acts as both an on/off switch to control when proteins are made and also a volume control that increases or decreases the amount of proteins made.

What are the steps of gene expression?

It consists of two major steps: transcription and translation. Together, transcription and translation are known as gene expression. During the process of transcription, the information stored in a gene’s DNA is passed to a similar molecule called RNA (ribonucleic acid) in the cell nucleus.

What are the key steps in gene expression?

How do you determine the expression level of a gene?

Most of these techniques, including microarray analysis and reverse transcription polymerase chain reaction (RT-PCR), work by measuring mRNA levels. However, researchers can also analyze gene expression by directly measuring protein levels with a technique known as a Western blot.

What are the two stages of gene expression?

What is the first step of gene expression?

Transcription
Transcription is the first step of gene expression. During this process, the DNA sequence of a gene is copied into RNA. Before transcription can take place, the DNA double helix must unwind near the gene that is getting transcribed. The region of opened-up DNA is called a transcription bubble.

What is normalized expression?

Normalization is achieved by dividing expression values by the total intensity (i.e., the sum of all expression values) of the given array. Centralization11 assumes that regulation is well behaved, i.e., most genes are not significantly regulated or about equal numbers of genes are up- and down-regulated.

What is pTPM?

A: Enrichment in tissues or regions, refers to RNA expression levels, whereas cell-type enrichment refers to proteins. A: The normalized expression (NX) is used to compare genes expression between tissues, while the protein-transcripts per million (pTPM) is used to compare between genes in the same tissue.