What is the role of Factor CstF?
CstF is recruited by cleavage and polyadenylation specificity factor (CPSF) and assembles into a protein complex on the 3′ end to promote the synthesis of a functional polyadenine tail, which results in a mature mRNA molecule ready to be exported from the cell nucleus to the cytosol for translation.
What is alternative polyadenylation?
Alternative polyadenylation (APA) is a widespread mechanism of gene regulation that generates distinct 3′ ends in transcripts made by RNA polymerase II. APA is tissue specific and globally regulated in various conditions, such as cell proliferation and differentiation, and in response to extracellular cues.
What is CPSF and CstF?
Cleavage and polyadenylation specificity factor (CPSF) binds to the AAUAAA signal, and cleavage stimulation factor F (CstF) binds to the G/U-rich signal.
What is RNA polyadenylation?
Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In many bacteria, the poly(A) tail promotes degradation of the mRNA.
What’s the main function of cleavage and polyadenylation specific factor CPSF )?
Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3′ signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription.
What are polyadenylation sites?
The polyadenylation site is the site of cleavage at which POLYA_TAIL is added in mRNA. It is localized downstream of the POLYA_SIGNAL. The POLYA_SITE can be determined by comparing cDNA and gDNA. The sequence at/or immediately 5′ to the site of RNA cleavage is frequently (but not always) CA.
What is polyadenylation Slideshare?
Polyadenylation is the addition of a poly(A) tail to a messenger RNA. The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation.
What does cleavage and polyadenylation do?
Cleavage and polyadenylation (pA) is a fundamental step that is required for the maturation of primary protein encoding transcripts into functional mRNAs that can be exported from the nucleus and translated in the cytoplasm.
What is the purpose of polyadenylation in bacteria?
Polyadenylation promotes the degradation of a regulatory RNA that inhibits the replication of bacterial plasmids and may play a similar role in the degradation of mRNA.
Which protein factor stabilizes the RNA interaction between CPSF and CstF in polyadenylation?
CPE-binding protein (CPEB) is an RNA-binding protein that binds to the CPE present in some mRNAs that controls polyadenylation. CPEB also interacts with symplekin, a scaffold-like protein that assembles other components involved in polyadenylation known as cleavage and polyadenylation specificity factor (CPSF).
What is the consequence of polyadenylation?
Implications of alterations in polyadenylation for endocrine disease: Alterations in polyadenylation have been found to be causative of neonatal diabetes and IPEX (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) and to be associated with type I and II diabetes, pre-eclampsia, fragile X-associated …
What specifies the site of polyadenylation?
The polyadenylation site is the site of cleavage at which POLYA_TAIL is added in mRNA. It is localized downstream of the POLYA_SIGNAL. The POLYA_SITE can be determined by comparing cDNA and gDNA.