How much DNA ladder should I use in PCR?
The 100bp DNA ladder is one of the commonly used standard molecular weight markers during the PCR. 2% agarose gel concentration is ideal for it, although, 2.5% concentration of gel gives more sharpen DNA bands (our own observations).
Why is my DNA ladder faint?
FAQ: Why are some of the bands in the DNA Ladder fainter at the bottom of the gel? Most stains run in the opposite direction from that of the migrating DNA. As a result, the bottom of the gel might have a lower concentration of stain, which might result in un-uniform staining of the bottom bands.
What is a DNA ladder standard marker used for?
A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel …
What is a 50 bp ladder?
Description: 50 bp DNA Ladder consists of 10 DNA fragments ranging from 50 bp to 500 bp and is supplied in ready-to-use format containing an orange tracking dye. It allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest.
What does bp mean in DNA?
Definition. Base pairs are the building blocks of the DNA molecule. The four base pairs include guanine, cytosine, adenine and thymine.
Why is the DNA ladder not separating?
When increasing percentage of low melting agarose gel (1.5% low melting or 1% low melting) the bands and ladder do not separate enough, when running on lower voltage such as 60-80V, for 2-3hours. Increasing voltage would melt the low-melting agarose.
What can a DNA ladder help determine?
DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories.