How long does a pollen tube take to grow?
For example, the maize pollen tube can grow as fast as 1 cm/h and extend to about 1 ft in length within 24 h (Barnabas and Fridvalszky, 1984). During the elongation process, the pollen cytoplasm, vegetative nucleus and sperm cells are transported within the tube, which grows through intercellular spaces in the pistil.
What promotes pollen germination tube growth?
Ionic elements, such as boron and calcium, that are involved in the metabolism during pollen tube growth, and at different concentrations can stimulate or inhibit it. Naturally occurring polyamines such as spermine also stimulate pollen tube germination and elongation when they are sprayed on the stigma.
Which type of sugar is best for pollen tube growth?
sucrose
Optimal sugar concentration for pollen germination was 300 g L–1, in which up to 81 % of pollen grains showed fast germination. Germination and emergence of multiple tubes were observed in sucrose concentrations of 100–800 g L–1.
How do you make a pollen germination slide?
Using a glass rod, stir the solution to mix it well. Using a dropper, take some nutrient solution and put two drops on a clean glass slide. Take a mature flower and dust a few pollen grains from its stamen on to the drop on the slide. After 5 minutes, place the glass slide on the stage of the compound microscope.
How do you measure the growth of a pollen tube?
The most common procedure is to measure and compare the lengths of individual pollen tubes. inhibition patterns. to the line of pollen, and their lengths can thus be easily measured. 24 C for about 20 hr and were then observed for tube growth.
Which element is important during pollen tube growth and essential for seed development?
The results of many studies indicate that calcium is a critical element that is strongly related to pollen germination and pollen tube growth.
Why Sucrose is used for pollen germination?
Why is sucrose used as a medium for pollen germination? In nature pollen grain germinates in response to a sugary fluid secreted by the mature stigma. Sucrose maintains the osmotic pressure to prevent the bursting and collapse of pollen grains.
Do sperm develop before or after pollination?
The tube cell (also referred to as the tube nucleus) develops into the pollen tube. The germ cell divides by mitosis to produce two sperm cells. Division of the germ cell can occur before or after pollination.
Why is sucrose used in pollen germination?
Which solution is used for pollen grain germination practical?
(i) Prepare the pollen germination medium by dissolving 10g sucrose, 30mg calcium nitrate and 10mg boric acid in 100ml of distilled water. Alternatively 10% sucrose solution can also be used. (ii) Take a drop of medium or 10% sucrose solution on a cover slip and sprinkle mature pollen grains on the drop.
What is pollen grain viability?
Pollen viability is the ability of pollen to effect fertilization and consequent development of fruit and seed; therefore fruit set and seed set form the most authentic test for pollen viability.
Which is the best concentration for pollen tube growth?
We can see from the graph that the most effective concentration for pollen tube growth was 0.4 mol dm-3 as the percentage of pollen cells with a tube formed was 36% compared to 30% of 0.2 mol dm-3. The experiment was done from 09:30 to 15:30, a period of 6 hours.
How long does it take a pollen tube to grow?
The attached ZIP file shows the growth of pollen tubes under the microscope over the course of 75 minutes.
How to prepare the medium for pollen germination?
Prepare the pollen germination medium by dissolving 10 grams sucrose, 10 milligrams boric acid, 30 milligrams magnesium sulphate and 20 milligrams potassium nitrate in 100ml of distilled water. Using a glass rod, stir the solution to mix it well. Using a dropper, take some nutrient solution and put two drops on a clean glass slide.
Why is it important to pick the right pollen?
Picking the right pollen for the right of year is important, and this is detailed in the ‘Pollen germination across the seasons’ resource. When a pollen grain is released from an anther, it is partially dehydrated.