How much does a Biacore cost?
Biophysics Services & Fees
| Service | Yale Cost per sample | Non-Yale* Cost per sample |
|---|---|---|
| BiaCore T100 | $300/day* | $850/day* |
| *All reservations for the SPR Service are for a 24 hour time slots from noon till noon. | ||
| Costs for Fluorescence Plate Reader | ||
| Plate Reader | $50.00/hour | $70.00/hour |
What is a CM5 chip?
The CM5 sensor chip carries a matrix of carboxymethylated dextran covalently attached to a gold surface. Molecules can be covalently coupled to the sensor surface by exploiting available amine, thiol, aldehyde, or carboxyl functional groups on the ligand.
What is Biacore technology?
The Biacore platform monitors molecular interactions in real time by using a non-invasive label-free technology. This technology reacts to changes in the concentration of molecules at the sensor surface as molecules bind to or detach from the surface.
How does Biacore work?
Biacore systems exploit the phenomenon of surface plasmon resonance (SPR) to monitor the interaction between molecules in real time. The approach involves attaching one interacting partner to the surface of a sensor chip, and then passing sample containing other interaction partner(s) over the surface.
What is Biacore testing?
SPR (Biacore) assays provide a method for determining the affinity and binding kinetics of a ligand for its receptor. Binding is measured as a change in resonance units (RUs) on the biosensor surface. Measuring the increase in binding over time for a given ligand concentration gives the association rate (ka or Kon).
What is BioCoRE used for?
BioCoRE facilitates individual and collaborative research and research management. Productive research relies on extensive and intensive interactions between scientists across disciplines and at different levels.
What is Amine Coupling?
Amine coupling is one of the most common methods to immobilize a protein or a small molecule containing primary amine group through a covalent bond on biosensors used in QCM(Quartz Crystal Microbalance), SPR(Surface Plasmon Resonance) or an electrode analysis.
What is SPR used for?
Surface plasmon resonance (SPR) binding analysis methodology is used to study molecular interactions (1, 2). SPR is an optical technique for detecting the interaction of two different molecules in which one is mobile and one is fixed on a thin gold film (1).
What is a two state reaction?
Two state reaction This model describes a 1:1 binding of analyte to immobilized ligand followed by a conformational or other change that. stabilizes the complex. To keep the model simple, it is assumed that the changed complex can only dissociate through. reversing the conformational change: A + B = AB = AB*
What is SPR sensor?
Surface plasmon resonance (SPR) sensor is now being utilized to detect the change of refractive index and, therefore, the mass density in accordance with molecular association or dissociation near the surface of thin metal layer.
What is EDC and NHS?
EDC (carbodiimide) crosslinking reaction scheme. Carboxyl-to-amine crosslinking with the popular carbodiimide, EDC. N-hydroxysuccinimide (NHS) or its water-soluble analog (Sulfo-NHS) is often included in EDC coupling protocols to improve efficiency or create dry-stable (amine-reactive) intermediates.
How do you name amides?
Primary amides are named by changing the name of the acid by dropping the -oic acid or -ic acid endings and adding -amide. The carbonyl carbon is given the #1 location number.
How are sensor chips used in Biacore systems?
This high-affinity streptavidin (SA) sensor chip immobilizes biotinylated molecules for SPR interaction analysis on Biacore systems. This high-affinity streptavidin (SA) sensor chip immobilizes biotinylated molecules for surface plasmon resonance (SPR) interaction analysis on Biacore systems.
What makes up the surface of the CM5 sensor chip?
The CM5 sensor chip carries a matrix of carboxymethylated dextran covalently attached to a gold surface. Molecules can be covalently coupled to the sensor surface by exploiting available amine, thiol, aldehyde, or carboxyl functional groups on the ligand.
How are Biacore systems used in real time?
1.1 Principles of Biacore systems Biacore systems exploit the phenomenon of surface plasmon resonance (SPR) to monitor the interaction between molecules in real time. The approach involves attaching one interacting partner to the surface of a sensor chip, and then passing sample containing other interaction partner(s) over the surface. Binding of