What antibiotic resistance does the pUC19 plasmid carry?
Plasmids pT7-7 and pUC19 carry an ampicillin-resistance gene (bla), pBR322 carries ampicillin- (bla) and tetracycline-resistance (tetA) genes, pCattTrE18 carries a chloramphenicol-resistance gene (cat), pACYC184 carries tetracycline- (tetA) and chloramphenicol-resistance (cat) genes, pACYC177 carries ampicillin- (bla) …
Is pUC19 antibiotic resistant?
In addition to β-galactosidase, pUC19 also encodes for an ampicillin resistance gene (ampR), via a β-lactamase enzyme that functions by degrading ampicillin and reducing its toxicity to the host.
What is the function of the bla gene in a plasmid vector?
bla — gene that encodes β-lactamase, an enzyme that breaks down the antibiotic ampicillin; transformants expressing the bla gene can be selected by placing ampicillin in the growth medium.
What genes carry antibiotic resistance?
A resistance plasmid is any plasmid that carries one or more antibiotic resistance genes (it may also be, for example, a metabolic plasmid, because it encodes a metabolic function, or a virulence plasmid, because it possesses one or more virulence genes.
Is pUC19 resistant to ampicillin?
The pUC19 plasmid confers ampicillin resistance to its host through its bla gene which codes for a β-lactamase that cleaves the β-lactam ring in ampicillin. The pUC19 plasmid also contains the lacZ gene which encodes the N-terminal fragment of the enzyme beta-galactosidase.
What are features of pUC19 vector?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
Which selection system is used in pUC19 plasmid?
triclosan selection
Vectors containing fabI enable triclosan selection. To test the potential of fabI as a selective marker for cloning, two vectors derived from pUC19 were constructed. First, we constructed pFab, where fabI together with its native promoter replaces the ampicillin resistance gene (bla) in pUC19.
What is gene encoding antibiotic resistance?
The evidence suggests that antibiotic resistance genes in human bacterial pathogens originate from a multitude of bacterial sources, indicating that the genomes of all bacteria can be considered as a single global gene pool into which most, if not all, bacteria can dip for genes necessary for survival.
How many genes are antibiotic resistant?
A total of 923 AR genes were identified from the sequences (Table 1).
What is pUC19 used for?
How many base pairs are there in pUC19?
The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
Why are there so many pUC18 / 19 plasmids?
pUC18/19 plasmids contain: 1. The pMB1 replicon rep responsible for the replication of plasmid (source – plasmid pBR322). The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in the replicon rep of pMB1.
How big is pUC18 compared to pUC19?
pUC18 / pUC19 from E.coli are commonly used small, high copy number, E. coli plasmids, 2686 bp in length. pUC19 is identical to pUC18 except that they contain multiple cloning sites (MCS) arranged in opposite orientations.
What is the copy number of pUC19 vector?
pUC19 is a common plasmid cloning vector in E.coli that is 2686 bp and has a high copy number. It carries a 54 bp multiple cloning site polylinker.