Which media is used to study cellulose degradation activity?
To indicate the cellulase activity of the organisms, diameter of clear zone around the colony and hydrolytic value on cellulose Congo Red agar media were measured.
How do you test for cellulase?
Screening for cellulase-producing microorganisms is routinely done on carboxymethylcellulose (CMC) plates. The culture plates are flooded either with 1% hexadecyltrimethyl ammonium bromide or with 0.1% Congo red followed by 1 M NaCl.
How do I prepare for CMC Media?
Preparation of carboxymethyl cellulose (CMC) from microcrystalline cellulose was carried out by an etherification process, using NaOH and monochloroacetic acid (MCA), with ethanol and water as the supporting medium.
Do bacteria produce cellulase?
Cellulase-producing bacteria were isolated from soil and identified as Pseudomonas fluorescens, Bacillus subtilIs, E. coli, and Serratia marcescens. Among bacteria, Pseudomonas fluorescens is the best cellulase producer among the four followed by Bacillus subtilis, E. coli, and Serratia marscens.
Which bacteria can degrade cellulose?
Microorganisms that can produce cellulase enzymes (cellulolytic microorganisms) can degrade cellulose. Fungi and bacteria isolated from soil secrete several enzymes which degrade lignocellulosic biomass [7].
What can produce cellulase?
Among bacteria, Pseudomonas fluorescens is the best cellulase producer among the four followed by Bacillus subtilis, E. coli, and Serratia marscens.
What kind of bacteria can cause contact lens conjunctivitis?
Etiology. Narrative reviews suggest that the causative agents of bacterial conjunctivitis and keratitis in contact lens wearers are usually gram-negative bacteria (such as Pseudomonas aeruginosa ), but may include all of the above agents. Acanthamoeba infections can be particularly difficult to diagnose and treat,…
How is cellulase produced by a microorganism?
Cellulases are the inducible bioactive compounds produced by microorganisms during their growth on Cellulosic matters (Lee and Koo, 2001). Cellulose degrading microorganisms can convert cellulose into soluble sugars either by acid and enzymatic hydrolysis.
What causes dilation of the conjunctival blood vessels?
Dilation of conjunctival blood vessels secondary to viral or bacterial infection, chemical exposures, or allergies results in the redness seen on the examination.
How long does it take for bacterial conjunctivitis to resolve?
Conjunctivitis resolves spontaneously without treatment within two to five days in more than one half of persons, but infectious complications rarely occur. Topical antibiotics may speed up clinical and microbiological cure of bacterial conjunctivitis, but the benefit is small.