What is API in LC-MS?
atmospheric pressure ionization (API).
What does LC-MS MS stand for?
Liquid Chromatography with tandem mass spectrometry
Liquid Chromatography with tandem mass spectrometry (LC-MS-MS) is a powerful analytical technique that combines the separating power of liquid chromatography with the highly sensitive and selective mass analysis capability of triple quadrupole mass spectrometry.
What is a LC-MS MS test?
Liquid Chromatography Mass Spectrometry (LC-MS) is a method or technique used commonly for drug analysis, food analysis, and environmental testing. Liquid Chromatography Mass Spectrometry combines the physical separation and detection sensitivity capabilities of both platforms.
What is the difference between LC and MS?
LC-MS and LC-MS/MS are the combination of liquid chromatography (LC) with mass spectrometry (MS). MS means you only analyze your precursor ion (as generated in the source) for example in an iontrap, a quadrupole or time-of-flight Massspec. MS/MS is the combination of two mass analyzers in one mass spec instrument.
Why is LC-MS better than GCMS?
The only difference is that LC-MS uses a solvent as its mobile phase, while GC-MS uses inert gases (like helium) in the same capacity. 3. GC-MS is the preferred standard for forensic identification, and it is also the preferred machine in terms of costs and operation.
What is the use of LC-MS?
LCMS is applied in many industries such as pharmaceuticals, biopharmaceuticals, forensic, industrial, food and environmental sector. For clinical research, the analysis of drugs, vitamins and minerals in whole blood, plasma, serum and urine is conducted routinely using LCMS.
How do LC-MS MS work?
LC/MS/MS utilizes liquid chromatography in a similar manner as described for HPLC. Following the chromatographic separation, molecules are ionized using an energy source. These ionized molecules then pass through a set of magnets, which further separates molecules based on their mass-to-charge ratio.
How much does a LC-MS cost?
As a rough approximation metals analyses usually run between $25 and $75 per sample, and LC/MS/MS and GC/MS/MS analyses are typically between $100 and $200 per sample. For lipidomics, the cost for running a quantitative analysis (targeted analysis of known lipids) is $120 per sample.
How does an LC-MS MS work?
What is the advantage of MS MS?
The MS/MS has three major advantages: The capacity to study numerous molecules regardless of whether they are from the same structural family or not; The capacity to highlight the specific metabolites of a disease; It’s an automated technique offering the possibility of large-scale analysis.
How accurate is LC-MS?
The assay also showed good accuracy, with intra-assay concentrations within 85.6% and 108.7% of the expected value, and inter-assay concentrations within 89.4 to 104.0% of the expected value.
Which is better GC-MS or LC-MS?
How does the API 4000 LC / MS / MS system work?
The instrument is configured to perform complex MS/MS analysis, but it can, for less rigorous analytic al requirements, perform single MS (LC/MS) scans. The API 4000 LC/MS/MS system allows all modes of MS/MS operation for full characterization of biopharmaceutical compou nds and the specificity needed for new drug development.
How does the AB SCIEX API 4000 LC / MS system work?
The AB SCIEX API 4000™ LC/MS/MS system uses the TurboIonSpray®probe and the atmospheric pressure chemical ionization (APCI) probe to produce ions from liquid samples. The term LC/MS/MS, applied to the triple quadrupole series, is a generic label for the combined analytical processes of liquid separation and subsequent mass spectrometric analysis.
Is the API 3200 mass spectrometer still in production?
The API 3200 LC-MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all in an affordable, compact package. The legacy API 3200 system is no longer in production, but may still be available as used or refurbished.
What is the Hardware Manual for the API 4000?
The API 4000™ LC/MS/MS System Hardware Manual is targeted to operators who are familiar with mass spectrometry but are new to the API 4000™LC/MS/MS system. This manual contains the instructions required to start up and operate the components of the API 4000 system.