What is FAM in PCR?
Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide synthesis and molecular biology.
Is 6-FAM the same as FAM?
Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein. The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
What is the wavelength of FAM?
493 nm
FAM (Carboxyfluorescein) is a fluorescent compound with an excitation peak at 493 nm and an emission peak at 517 nm. It can be excited using a 488 nm laser paired with a 530/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.
What is FAM fluorophore?
A single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5.
What is FAM and Vic?
A popular combination of dyes is FAM or VIC for the reporter dye and TAMRA for the quencher dye. In this case, the IPC is labeled with a VIC (green) reporter dye and hybridizes to a synthetic template added to each reaction.
WHAT ARE FAM and HEX probes?
Four differently colored fluorophores (FAM: green; HEX: orange; ROX: red; and Cy5: blue) and one universal quencher (DABCYL: black) are used to depict the principal of MCPC. Four probes, each labeled with a different single color. C) Six probes, each labeled with a unique combination of two of the four colors.
Is Tamra a quencher?
TAMRA dye is an effective quencher for fluorophores with emission maxima less than 560 nm. Dyes with longer wavelength emissions will not be effectively quenched by TAMRA. In addition, TAMRA has its own fluorescence which complicates data analysis due to crosstalk between the channels.
Is FAM the same as FITC?
IDT offers a 6-FAM fluorophore modification instead of the traditional FITC fluorophore modification. FITC and FAM are both derivatives of fluorescein. FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM is 6-carboxyfluorescein. FITC and 6-FAM have identical spectral characteristics.
What color is Alexa Fluor 680?
red
About Alexa Fluor 680 Alexa Fluor® 680 (AF680, Alexa 680) is a red-emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 695/40 nm bandpass filter.
Is Alexa Fluor 488 FITC?
Molecular Probes™ Alexa Fluor® 488 dye—with nearly identical spectral properties and quantum yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.
ARE FAM and FITC the same?
FITC and FAM are both derivatives of fluorescein. FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM is 6-carboxyfluorescein. FITC and 6-FAM have identical spectral characteristics. In experiments, 6-FAM performs exactly like FITC.
Is FAM a reporter dye?
Figure1 provides a list of reporter dyes compatible with common instrumentation. FAM is the most popular of these dyes, and often provides greater assay sensitivity than some of the other dyes available.
What is the absorption wavelength of 6 FAM?
6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm.
What is 5’6 FAM return to fluorophores modification?
5′ 6-FAM (Fluorescein) Return to Fluorophores Modifications A single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment.
Which is correct 5 FAM or 6 FAM?
Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5 (6)-carboxyfluorescein. The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading. It can be incorporated into liposomes, and allows for the tracking of liposomes as they pass through the body.
What is the emission peak of carboxyfluorescein?
FAM (Carboxyfluorescein) is a fluorescent compound with an excitation peak at 493 nm and an emission peak at 517 nm. It can be excited using a 488 nm laser paired with a 530/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.