What is Quantikine ELISA Kit?

What is Quantikine ELISA Kit?

Quantikine Kits are complete, fully validated, ready-to-run sandwich ELISAs that are designed to provide the highest levels of specificity, accuracy, precision, and sensitivity in analyte quantification.

What is Quantikine?

Quantikine ELISAs are based on a two-site sandwich principle in which two highly specific antibodies are used to detect the target analyte. Quantikine Kits provide a 96-well microplate pre-coated with a capture antibody specific for the analyte of interest.

How do you run an Elisa?

Steps to run a sandwich ELISA assay

1. Step 1: Capture antibody binds to ELISA plate wells.
2. Step 2: Add sample to well – antigen within the sample binds to the capture antibody.
3. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest.

What is a DuoSet Elisa?

DuoSet ELISA Development Systems contain the basic components required to develop a sandwich immunoassay for accurately measuring analytes in biological fluids. When complete kits are not an option, DuoSet ELISA Development Kits are an economical alternative to buying separate antibodies and protein standards.

What is the principle of Elisa?

Principle of ELISA ELISA works on the principle that specific antibodies bind the target antigen and detect the presence and quantity of antigens binding. In order to increase the sensitivity and precision of the assay, the plate must be coated with antibodies with high affinity.

How do you dispose of ELISA reagents?

Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Dispose of as unused product.

What is an assay diluent?

Assay Diluents are additive components used in an ELISA to equalize any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve.

What are the 4 steps of ELISA?

The Direct ELISA Procedure can be summarised into 4 steps: Plate Coating, Plate Blocking, Antibody Incubation, and Detection.

How do I make an ELISA kit?

1. ELISA Coating Strategy. When developing a new ELISA for a specific antigen, the first step is to determine an immobilizing strategy and optimize the plate-coating conditions for the antigen or capture antibody.
2. Antigen and Antibody.
3. Conjugate Strategy.
4. Enzyme and Chromogen.

What is Elisa sandwich?

The sandwich ELISA is a type of Enzyme-linked immunosorbent Assay that uses two antibodies: a capture antibody and a detection antibody. The purpose of any ELISA is to detect the presence of a target antigen in a sample. This means that they are known to bind to different places on the target antigen. …

How do you make a reagent diluent for Elisa?

Reagent Diluent Concentrate 1 Summary Dilute 1.4 mL to a final volume of 100 mL with PBS + 0.05% Tween® 20, pH 7.2-7.4. Use the diluted reagent within 8 hours.

What can a quantikine ELISA kit be used for?

R&D Systems Quantikine ELISA Kits are complete and ready-to-use. Immunoassays are available for measuring a range of molecules including cytokines, chemokines, growth factors, proteases, and more. R&D Systems Quantikine ELISA Kits – Your Blueprint to Success!

How long has are & D systems been developing Elisa?

R&D Systems has been developing ELISA kits for over 30 years. We have a wide range of assays available, from our Quantikine colorimetric ready-to-use kits to our DuoSet Development ELISAs, spanning 12 different species and over 700 different targets.

What are the results of the IL-6 quantikine kit?

Results obtained using natural IL-6 showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-6.

How are ELISA kits used in a range of formats?

Quantikine ELISA kits are available in a range of formats for measuring intracellular and extracellular proteins. Our master calibrated Quantikine assays ensure that our kits perform consistently over time and are optimized to mitigate matrix effects and avoid false-positive results.