Is cisplatin soluble in DMSO?
Cisplatin is soluble in organic solvents such as DMSO* and dimethyl formamide (DMF), which should be purged with an inert gas. It is also soluble in water. The solubility of cisplatin in DMF is approximately 10 mg/ml and approximately 1 mg/ml (with warming) in water.
How do you dissolve cisplatin in PBS?
All Answers (4) You can dissolve cisplatin in deionized water by vortexing it real well, but do not go higher than 2.5 mM, which is the limit. Before use, let the solution into a shaker for 10 min at RT.
What do I give cisplatin with?
Hydration is recommended to induce diuresis and reduce the concentration of cisplatin in the kidneys. Isotonic saline (0.9% sodium chloride) is recommended for hydration fluids, and many centers also include potassium and magnesium in these hydration fluids due to the risk of electrolyte wasting.
How do you dilute cisplatin?
In conclusion, cisplatin appears to be physically and chemically stable for at least 30 days in concentrate in glass containers or diluted with 0.9% sodium chloride in PE bags at a concentration of 10 mg/ml, at room temperature (15–25°C) when protected from light.
How do you reconstitute cisplatin?
Reconstitution. Reconstitute 50 mg vials with 50 mL of Sterile Water for Injection, USP. Each mL of the resulting solution will contain 1 mg of cisplatin for injection. Reconstitution results in a clear or colorless to slight yellow solution.
How do you make cisplatin solution?
A cisplatin solution in a concentration of 0.1 mg/ml was also analysed. The solution was prepared by adding a concentrate of 1 mg/ml to a 0.9% NaCl solution and storing at the temperature of 21.7 ±1.3°C without access to light.
Can cisplatin be refrigerated?
Cisplatin Injection is a sterile, multidose vial without preservatives. Store at 15° to 25°C (59° to 77°F). Do not refrigerate.
How can I reduce the side effects of cisplatin?
Drug therapy is used to prevent or relieve nausea and vomiting. Not eating before your treatment may help relieve vomiting. Changes in diet such as eating several small meals or limiting activity may help lessen some of these effects. If any of these effects persist or worsen, notify your doctor or pharmacist promptly.
How do you store cisplatin?
Store at 15° to 25°C (59° to 77°F). Do not refrigerate. Protect unopened container from light. The cisplatin remaining in the amber vial following initial entry is stable for 28 days protected from light or for 7 days under fluorescent room light.
Is cisplatin compatible with dextrose?
Do not dilute cisplatin in just 5% Dextrose Injection. Adequate hydration and urinary output must be maintained during the following 24 hours. The infusion solution should have a final sodium chloride concentration ≥0.25%.
What’s the difference between DMSO and cisplatin in platinum?
Assessment of alternative solvent systems for platinum complexes. One limitation of aqueous formulations of cisplatin is its maximal solubility of just more than 3 mmol/L, whereas DMSO solutions can be prepared of more than 10 mmol/L. We examined whether other organic solvents had a similar effect on cisplatin.
Why is cisplatin used in a saline solution?
For this reason, cisplatin is formulated for clinical use in saline solution with a high chloride concentration (154 mmol/L) to prevent drug aquation before administration, stabilizing the drug and preventing side reactions before cell entry.
How does DMSO change the structure of platinum complexes?
DMSO contains a nucleophilic sulfur, which allows it to coordinate with platinum complexes, displacing ligands and changing the structure of the complexes ( 13–16 ). This renders platinum complexes unstable in DMSO.
How is DMSO used to treat osteosarcoma in dogs?
Massart and colleagues first reported that DMSO reduced cisplatin’s cytotoxicity toward cultured thyrocytes ( 17 ), and Dernel and colleagues reported that a polymer-based drug delivery system limited activity of cisplatin against stage IIb appendicular osteosarcoma in dogs ( 18 ).