What is the difference between spread and pour plating methods?
The key difference between Pour plate and Spread plate is that a known volume of the sample is spread on the surface of the agar medium in spread plate, while, in pour plate, a known volume of the sample is mixed with agar and then poured into a plate.
What is dilution plate technique?
Dilution plating is a simple technique used to estimate the number of heterotrophic bacteria in an environmental sample. Dilution plating is based on serial dilution of the sample. Serial dilution involves repeatedly mixing known amounts of source sample or culture with sterile liquid, usually a low-molarity buffer.
Why are serial dilutions used with both the pour plate and spread plate methods of plating?
Why are serial dilutions used with both the pour plate and spread plate methods of plating? Using serial dilutions makes it possible to identify the nonliving cells in the original sample. Without dilution most of the bacteria would die before the colonies formed on the agar.
Why We Do dilution in plate count method?
We use serial dilutions to create decreasing concentrations of the original sample that are then plated so that a plate will be created with a low enough number of bacteria that we can count individual colonies.
What is pour plate and spread plate?
Definition. Pour Plate: A plate prepared by mixing the inoculum with the cooled but still molten medium before pouring the latter into the Petri dish. Spread Plate: A technique used to count or isolate bacterial colonies on the surface of the agar.
What is spread plate method?
Spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly. The technique makes it easier to quantify bacteria in a solution.
What is serial dilution and why is it used?
A serial dilution is the stepwise dilution of a substance in solution. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale.
What is pour plate method?
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plate. Each colony represents a “colony-forming unit” (CFU).
How do you do the spread plate method?
Sterilize a plate spreader and touch it to the surface of the agar, away from the dispensed liquid, to cool. To spread the dispensed liquid around the plate, gently push the plate spreader back and forth. Use your other hand to hold the lid above the plate and to spin the plate to get an even spread.
How do you do a spread plate?
What is spread plate?
The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
How do you do a pour plate technique?
The pour-plate technique Molten agar cooled to 45°C, is poured into a Petri dish containing a specified amount of the diluted sample. Following the addition of the molten-then cooled agar, the cover is replaced, and the plates gently rotated in a circular motion to achieve uniform distribution of microorganisms.
How is the spread plate method used in microbiology?
Spread Plate Technique A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures. The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate.
What’s the difference between a spread plate and a pour plate?
Pour plate technique is a microbial method to enumerate some viable cells present in a sample. The speciality of the pour plate method is that a known volume of the sample is first mixed with agar and then poured into the plate. Other steps are similar to the spread plate technique discussed in the next section.
Which is the speciality of the pour plate method?
Pour plate technique is a microbial method to enumerate some viable cells present in a sample. The speciality of the pour plate method is that a known volume of the sample is first mixed with agar and then poured into the plate.
How are serial dilutions recorded in a plate count?
Generally replicates of each dilution are plated, and the mean count recorded. Spread Plate Method Another technique for performing a standard plate count is the spread plate method. As the name implies, serial dilutions of a sample are spread onto the surfaces of agar plates.