What is FluoroGold used to identify?
Virtually any central or peripheral nervous system structure can be injected with Fluoro-Gold for analysis of retrograde transport. In the peripheral nervous system, ganglia and peripheral targets can be studied.
What color is FluoroGold?
Product Attributes
| CAS number | 223769-64-0 |
|---|---|
| Probe cellular localization | Fluid phase tracer |
| Cell permeability | Membrane impermeant |
| Colors | Green |
| Excitation/Emission | 361/536 nm |
How do you dilute FluoroGold?
FluoroGold™ can be dissolved in 0.9% saline or distilled water.
How do Retrobeads work?
Retrobeads™ IX: Retrobeads™ deliver bioactive agents (such as neurotrophins and neurotransmitter agonists/antagonists) to localized regions; retrograde transport allows determining which neurons were exposed to the agents [Riddle et al.
What are neuronal tracers?
Neuronal tracing, or neuron reconstruction is a technique used in neuroscience to determine the pathway of the neurites or neuronal processes, the axons and dendrites, of a neuron. Viral neuronal tracing, for a technique which can be used to label in either direction; Manual tracing of neuronal imagery.
How do retrograde tracers work?
Retrograde tracing (retrograde labeling) is a neuroanatomical method used to determine the location of the cells of origin of a nervous system pathway. A tracer substance that will be taken up by synaptic terminals (and sometimes by axons) is injected into a region of interest, such as a central nervous system nucleus.
What is anatomical tracing?
From Wikipedia, the free encyclopedia. In neuroscience, anterograde tracing is a research method which is used to trace axonal projections from their source (the cell body or soma) to their point of termination (the synapse).
What is anterograde and retrograde tracing?
Anterograde tracing outlines neurons from their cell bodies to the terminals of their axons; while retrograde tracing outlines neurons in the opposite direction, from the terminals of their axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport pathways in neurons.
How is anterograde tracing different from retrograde tracing?
How is a fluorophore excited by a wavelength?
A fluorophore is excited most efficiently by light of a particular wavelength. This wavelength is the excitation maximum for the fluorophore. Light with a wavelength near the excitation maximum can also cause excitation, as shown by the shaded areas below, but it does so less efficiently.
How does the excitation maximum affect the fluorescence output?
It is important to remember that although illumination at the excitation maximum of the fluorophore produces the greatest fluorescence output, illumination at lower or higher wavelengths affects only the intensity of the emitted light—the range and overall shape of the emission profile are unchanged.
Which is the best wavelength to detect fluorochrome?
When excited by 488-nm light, the excited fluorochrome (PE) is able to transfer its fluorescent energy to the cyanine molecule, which then fluoresces at a longer wavelength. The resulting fluorescent maximum is approximately 670 nm Using a 650-nm longpass filter will give optimum detection for this fluorochrome.
Which is the best filter for fluorochrome absorption?
APC has 6 phycocyanobilin chromophores per molecule, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. It has a 650 nm wavelength absorption maximum and a 660 nm fluorescence emission maximum. Using a 660 ± 10 nm BP filter will give optimum detection for this fluorochrome.