Which is the optimal repair pathway for DSB?

Which is the optimal repair pathway for DSB?

For the precise repair of DSBs, the repair pathway that is optimal for the particular circumstance needs to be selected. Non-homologous end joining (NHEJ) functions in G1/S/G2 phase, while homologous recombination (HR) becomes active only in S/G2 phase after DNA replication.

What are DNA repair pathways?

DNA repair pathways are triggered to maintain genetic stability and integrity when mammalian cells are exposed to endogenous or exogenous DNA-damaging agents. The deregulation of DNA repair pathways is associated with the initiation and progression of cancer.

What is DSB repair?

To study DSB repair using this system, researchers treat cells with a dose of IR high enough so that the genomic DNA is shattered, leaving no intact chromosomal bands. Cells are then allowed time to recuperate before their DNA is extracted and resolved by PFGE.

What is the other name of DSB repair pathway?

What is the other name of DSB repair pathway? Explanation: The major DSB repair pathway is also known as the RecBCD pathway. Homologous recombination in eukaryotic cells and the proteins are involved in this pathway.

How can DNA breaks be repaired?

DNA double-strand breaks are repaired by means of two main mechanisms: nonhomologous end joining and homologous recombination (see Figure 1). Homologous recombination is the preferred route in yeast. It involves invasion of the broken DNA strands into a homologous DNA duplex molecule.

What is the proper order of the steps involved in excision repair?

Terms in this set (26) What is the proper order of the steps involved in excision repair? Recognize the damage, resynthesize the sequence, remove the damage, ligate the DNA backbone. Recognize the damage, remove the damage, resynthesize the sequence, ligate the DNA backbone.

Which DNA is connected by Holliday junction?

Holliday junction, cross-shaped structure that forms during the process of genetic recombination, when two double-stranded DNA molecules become separated into four strands in order to exchange segments of genetic information.

Which is the correct pathway to repair a DSB?

Frank DSBs, such as those produced by nucleases and IR, can be repaired by either pathway. DSBs produced by replication fork collapse are repaired primarily (or perhaps exclusively) by HR 1, 13. Note that fork collapse produces a one-ended DSB, better described as a “double-strand end” (DSE).

How are DSBs repaired by NHEJ and HR?

DSBs are repaired by non-homologous end-joining (NHEJ) and homologous recombination (HR), and defects in these pathways cause genome instability and promote tumorigenesis.

How is the choice of a repair pathway controlled?

Repair pathway choice may be controlled by the early acting proteins that influence both repair pathways. Once the commitment is made to a repair pathway, pathway-specific proteins drive the reaction toward HR or NHEJ products.