What is YPet?
YPet is a basic (constitutively fluorescent) yellow fluorescent protein published in 2005, derived from Aequorea victoria. It has moderate acid sensitivity.
What is the difference between YFP and GFP?
The main difference between GFP and YFP is that GFP exhibits green color upon exposure to light ranges from blue to ultraviolet whereas YFP exhibits yellow color upon exposure to the same light. Furthermore, GFP is originally derived from the jellyfish, Aequorea Victoria while YFP is a genetic mutant of GFP protein.
What is YFP and CFP?
Green fluorescent protein (GFP) is a bioluminescent polypeptide protein that is extracted from jellyfish (Aequorea victoria). On contrary, recently generated mutants of GFP with yellow-shifted (YFP) and cyan-shifted (CFP) emission spectrum are highly stable and brighter, when compared to BFP.
What is yellow fluorescent protein used for?
They have reduced chloride sensitivity, faster maturation, and increased brightness (defined as the product of the extinction coefficient and quantum yield). Typically, YFP serves as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is monomeric cyan fluorescent protein (mCFP).
What is Venus fluorescent?
Venus is a basic (constitutively fluorescent) yellow fluorescent protein published in 2002, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.
What is CFP and YFP?
Abstract. The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking and tracing of specific proteins or cells or to determine protein interactions.
Can I use CFP and GFP?
These results demonstrate that GFP and CFP fluorescence can be successfully separated even when simultaneously excited using an 880 nm laser beam. Furthermore, specific sub-cellular compartments or proteins can be visualized in cells simultaneously expressing GFP and CFP.
What color is CFP?
Cyan (CFP) color variants that are extracted from the Aequorea GFP are considered to be a perfect alternative to BFPs. Cyan fluorescence exists as a result of substitution of Tyr66 with tryptophan.
What kind of fluorescent protein is Ypet made of?
YPet is a basic (constitutively fluorescent) yellow fluorescent protein published in 2005, derived from Aequorea victoria. It has moderate acid sensitivity.
What happens when yfp3 is combined with YPet?
In an attempt to further enhance YFP3brightness, the Venusmutations were combined with those of YFP3to yield YFP for energy transfer (YPet). Though YFP3exhibited slower folding relative to Venus, combining YFP3and Venusmutations resulted in intermediate folding kinetics.
Where does YPet get its amino acids from?
YPet was derived from Venuswith the following mutations: I48L/L69V/S209F/V225L/L232E/D235NI47L/L68V/S208F/V224L/L231E/D234N amino acid numbers relative to VenusavGFP. show relative to avGFPshow relative to Venus