What is a cellular target?
A target cell is a particular cell with attached receptors to which a biochemical agent such as a hormone binds together, inducing a physiological reaction in the body.
What is target engagement assay?
Target engagement (TE) assays measure compound binding at select target proteins in intact cells. These assays also provide information about compound-target residence time and can be used to screen a panel of inhibitors to compare IC50 values.
What is a NanoBRET assay?
The NanoBRET™ assay is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc® Luciferase as the BRET energy donor and HaloTag® protein labeled with the HaloTag® NanoBRET™ 618 fluorescent Ligand as the energy acceptor to measure the interaction of two binding partners in live cells.
Can I return a Consumer Cellular phone to target?
Target does accept returns of cell phones purchased in-store or online within 14 days after the purchase or online order pickup (there are no exceptions to this policy). You can also return cell phones without the receipt if you can provide an alternative proof of purchase.
What is a Target Mobile kiosk?
Target Mobile is a “convenient and simplified cell phone shopping experience both in-store and online.” The bullseyed logoed company announced that by the end of this year (2010 for those of you not keeping track), 850 stores will have these mobile centers in their stores.
What are target engagement biomarkers?
Target engagement (TE): these biomarkers measure the percentage of the target protein that is bound by the drug. They are extremely useful to establish a direct link between PK and efficacy.
How do you measure target engagement?
Target engagement can be measured by immunoprecipitation of the kinase of interest from cells treated with a chemical probe (or vehicle control), followed by SILAC-LC-MS/MS to quantify changes in the phosphorylation status of autophosphorylated residues.
How do BRET assays work?
BRET, or Bioluminescence Resonance Energy Transfer, is a cell-based assay for studying protein-protein interactions. BRET relies on Forster resonance energy transfer from a bioluminescent donor to an acceptor in the presence of a substrate. Interestingly, BRET is a naturally occurring phenomenon in sea pansy.
What is a HiBiT assay?
Detect low-abundance proteins The HiBiT assay is based on bioluminescence, which is more sensitive than fluorophore-based methods, such as GFP. This allows detection at extremely low levels. Not only is it sensitive, it is also highly quantitative.
What is Target’s return policy for cell phones?
We make phone returns simple. Just bring it back to the cell phone activation counter at any store within 14 days of pickup. The carrier account holder must be present with a valid ID. (We don’t currently allow cell phones to be returned by mail.)
Are Consumer Cellular phones returnable?
With Consumer Cellular’s “Risk-Free” guarantee, you can return your phone, cancel your service, and pay nothing, provided you do so within 30 days of the activation of your service.
Does Target activate cell phones?
If you’d like to use your new cellphone right away, a store associate at Target can activate your phone so you’ll be able to call, text and more when you leave the store. You can also choose to activate it yourself, whenever you’re ready, simply by calling us at: 1-888-345-5509.