What is zorbax column?
The Agilent ZORBAX column family is one of the most popular HPLC column families for reversed-phase HPLC. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution.
What is NH2 column?
Spherisorb NH2 (Amino) columns contain a silica-based aminopropyl bonded sorbent. The column is listed as USP L8 and is used for anion-exchange, normal-phase and HILIC separations. Part Number: PSS830079. Chemistry. Amino (NH2)
Who makes zorbax columns?
Agilent Technologies Inc.
Agilent’s ZORBAX columns are optimized for high throughput analyses. Zorbax spherical particles are manufactured by Agilent Technologies Inc. from small, extremely uniform colloidal silica microbeads.
What is ODS and BDS column?
ODS and BDS are two columns used for reverse-phase chromatography. The key difference between ODS and BDS column is that ODS column contains free –OH functional groups, whereas BDS column contains deactivated –OH groups. Moreover, ODS columns have high peak tailing while BDS columns are designed to reduce peak tailing.
What is the structure of NH2?
If we look at the Lewis Structure of NH2- ion, we can see that we have four electron-rich regions around our central nitrogen atom. There are two bonded electron pairs around N to form single covalent bonds each with a Hydrogen atom on either side.
What is Agilent column?
Agilent J&W GC columns help lab analysts maintain the highest standards of performance. The columns deliver industry-leading technology with the highest inertness, lowest bleed levels, and tightest column-to-column reproducibility specifications.
Why is ODS used?
An operational data store (ODS) is a central database that provides a snapshot of the latest data from multiple transactional systems for operational reporting. It enables organizations to combine data in its original format from various sources into a single destination to make it available for business reporting.
What is Endcapping in column?
In chromatography, endcapping refers to the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. End-capped columns have much lower residual silanol group activity compared to non-endcapped columns.
What is the difference between H2N and NH2?
NH2 and H2N are the same.
What do you use Agilent zorbax NH2 for?
Agilent ZORBAX NH2 is an amino-propyl silane phase bonded to ZORBAX SIL. It is used for normal-phase and weak anion-exchange as well as reversed-phase HPLC of polar compounds. Vitamins A and D are separated in the normal phase mode. Carbohydrates and sugars are separated in the reversed-phase mode.
Which is the best zorbax column for HPLC?
ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. From ZORBAX Eclipse Plus C18 to sub-2 micron UHPLC columms, a wide selection of stationary phases and particle sizes allows you to fine-tune your selectivity to match your application.
What makes zorbax bonus RP columns so special?
ZORBAX Bonus RP columns offer unique selectivity due to an embedded amide linkage in the C14-alkyl chain making them ideal for 100% aqueous mobile phases and for compounds that are not retained on standard reverse phase columns ZORBAX Rx columns are made from highly pure (> 99.995 %) porous silica with a higher carbon load (12%)
What are the advantages of Agilent zorbax columns?
ZORBAX Rx columns are made from highly pure (> 99.995 %) porous silica with a higher carbon load (12%) The Agilent ZORBAX family offers all advantages of totally porous particle columns such as increased retention, loadability and resistance to sample solvents.