What is a Biotyper?

What is a Biotyper?

The MALDI Biotyper® (MBT) is a microbial identification system based on MALDI-TOF mass spectrometry, allowing unbiased identification of microorganisms within a few minutes down to the species level. Continuous expansion of the reference library ensures that a broad range of microorganisms can be identified easily.

How does MALDI-TOF mass spectrometry work for bacterial identification?

MALDI-TOF measures the mass of molecules from a sample that has been embedded in a matrix by using a laser to ablate and desorb the molecules with minimal fragmentation.

What is a MALDI score?

The MALDI Biotyper 2.0 software compares the peak profile of tested strains with database entries and calculates a logarithmic score between 0 and 3, which quantifies the similarity to the database entries. High values in this score represent high similarity with respective database entries.

What is the principle of Maldi-Tof?

MALDI is the abbreviation for “Matrix Assisted Laser Desorption/Ionization.” The sample for MALDI is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy.

What is Maldi-TOF mass spectrometry?

MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry is a new technology that has revolutionized pathogen identification and has also proven to accelerate detection of antimicrobial resistance compared to the traditional antibiotic susceptibility tests (AST) as well as DNA …

Why is maldi used?

Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.

How do you clean a maldi plate?

Cleaning of the sample plate

1. Use a stainless steel or gold MALDI sample plate.
2. Wash with MeOH and wipe gently with a Kimwipe.
3. Wash with H2O and wipe gently with a Kimwipe.
4. Wash with MeOH and wipe gently with a Kimwipe.
5. If needed, repeat MeOH/H2O/MeOH cleaning cycle, always ending with MeOH.

What kind of mass spectrometry does Bruker use?

By using our experience and expertise as a worldwide leading provider of MALDI-TOF mass spectrometry, Bruker developed the MALDI Biotyper ® – a revolutionary approach for the analysis of microorganisms. This “Open Microbiology Concept” includes interfacing, own library creation and can be easily integrated into any laboratory environment.

What kind of MRMS technology does Bruker use?

Bruker’s scimaX® and solariX series instruments are powered by MRMS (Magnetic Resonance Mass Spectrometry) technology. Compared with common FTMS technologies, these instruments feature unmatched eXtreme Resolution and mass accuracy.

What kind of laser system does Bruker use?

The NEW MALDI Biotyper ® sirius one GP or RUO System, with Bruker´s proprietary lifetime * smartbeam™ solid state laser technology at 200 Hz repetition rate and positive ion mode. System improvements, including the newest electronics and high-performance vacuum system, generate very fast target exchange.

How is Bruker working on single cell proteomics?

Bruker’s collaborators are making major progress in unbiased single cell proteomics, phosphoproteomics and plasma proteomics, leveraging the speed, sensitivity and dynamic range of large-scale CCS-enabled 4D-Proteomics and 4D-Epiproteomics.

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