What is the function of PAS stain?
The periodic acid-Schiff (PAS) staining procedure is most commonly used in the histology laboratory to detect glycogen deposits in the liver when glycogen storage disease is suspected. Glycogen granules may also be visible in tumors of the bladder, kidney, ovary, pancreas, and lung.
What is stain in liver?
Masson’s trichrome stain is among the most common special stains applied to liver specimens. The stain imparts a blue color to collagen against a red background of hepatocytes and other structures.
What is the purpose of glycogen in the liver?
Liver glycogen stores serve as a store of glucose for use throughout the body, particularly the central nervous system. The human brain consumes approximately 60% of blood glucose in fasted, sedentary individuals. Glycogen is the analogue of starch, a glucose polymer that functions as energy storage in plants.
What is responsible for the pink staining of hepatocytes?
In paraffin sections of liver stained with hematoxylin and eosin, accumulations of glycogen in hepatocytes do not stand out. However, when stained with using the periodic acid-Schiff (PAS) technique, glycogen stains bright pink in color.
What is periodic acid Schiff with Diastase?
Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease.
What does hematoxylin stain?
Introduction. The H&E stain provides a comprehensive picture of the microanatomy of organs and tissues. Hematoxylin precisely stains nuclear components, including heterochromatin and nucleoli, while eosin stains cytoplasmic components including collagen and elastic fibers, muscle fibers and red blood cells.
What stain detects fatty degeneration?
Fatty degeneration: In this condition, the accumulation of lipids occurs within the cell. The lipid accumulation can be observed by staining it with Sudan black or Osmium tetroxide.
What is the function and importance of glycogen?
Glycogen is an important energy store for the body. Liver glycogen, for example, plays a role in regulating blood sugar levels and homeostasis. On the other hand, muscle glycogen helps in the contraction of skeletal muscle and thereby physical activity.
What is the major physiological purpose of glycogen?
A main function of glycogen is to maintain a physiological blood glucose concentration, but only liver glycogen directly contributes to release of glucose into the blood.
What is the function of hepatocyte?
Hepatocytes, the major parenchymal cells in the liver, play pivotal roles in metabolism, detoxification, and protein synthesis. Hepatocytes also activate innate immunity against invading microorganisms by secreting innate immunity proteins.
What is the function of hepatocytes quizlet?
protein synthesis, and Bile synthesis.
What is the color of glycogen in hepatocytes?
In paraffin sections of liver stained with hematoxylin and eosin, accumulations of glycogen in hepatocytes do not stand out. However, when stained with using the periodic acid-Schiff (PAS) technique, glycogen stains bright pink in color.
What is the role of Special stains in diagnostic liver pathology?
The stain imparts a blue color to collagen against a red background of hepatocytes and other structures. It stains type 1 collagen that is normally present in the portal tracts and vessel walls, but also highlights the presence and distribution of reactive fibrosis as a result of liver injury.
How is glycogen released from the pancreas to the liver?
In the liver cells, the glycogen makes up to 6-10% of the liver by weight. If the food taken is not digested, then the blood glucose level increases and the insulin are released from the pancreas promoting the uptake of glucose into the liver cells. The enzymes involved in the glycogen synthesis are activated by the insulin.
What is the role of the liver in protein synthesis?
Protein synthesis is the process whereby the body’s cells make proteins that are necessary for cell structure and function. Liver cells play a role in protein synthesis and amino acid metabolism, and is involved in the production of ammonia.