What is the restriction site of EcoRI enzyme?

What is the restriction site of EcoRI enzyme?

Additional Information: The EcoRI is a restriction enzyme that creates four nucleotide sticky ends with the end of 5′. The enzyme cuts at the recognition site of G/AATTC which has a complementary sequence of CTTAA/G.

What is the recognition site for EcoRI?

EcoRI ligation. The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5′-GAATTC’-3, and introduces a single-strand cut between the G & A nucleotides. This recognition site is a palindrome: the opposite strand also reads 5′-GAATTC’-3 and will be cut in the same manner.

How many restriction sites are there for EcoRI?

Note, after a reaction with the EcoRI enzyme, that the DNA of species A is cleaved into three fragments, corresponding to two EcoRI restriction sites, whereas that of species B is cleaved into four fragments, corresponding to three EcoRI restriction sites.

What is the recognition site for Hind 2?

Hind II recognizes the sequence GTPy/PuAC and generates fragments with blunt ends (1). Compatible ends Hind II generates fragments with blunt ends and is compatible to any other blunt end. Isoschizomers Hind II is an isoschizomer to Hinc II.

What is a palindrome site?

Palindrome: In genetics, a DNA or RNA sequence that reads the same in both directions. The sites of many restriction enzymes that cut (restrict) DNA are palindromes.

What is the restriction site for HindIII?

Thermo Scientific HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

How many restriction sites do plasmids have?

A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites – a standard feature of engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid.

How many restriction sites are there in pbr322?

There are six key restriction sites inside the AmpR gene. The circular sequence is numbered such that 0 is the middle of the unique EcoRI site and the count increases through the TetR gene.

Does Hind 3 produce blunt ends?

Option B: Hind 3: It is a type 2 restriction endonuclease which gives sticky ends. It is isolated from Haemophilus influenzae. Eco RV: It is type 2 endonuclease producing blunt ends in the centre of nucleotide sequence GAT/ATC.

Which type of restriction enzyme is Hind 2?

HindIII (pronounced “Hin D Three”) is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.

Are restriction sites palindromic?

Restriction-modification systems are used as a defensive mechanism against inappropriate invasion of foreign DNA. The recognition sequences for the common type II restriction enzymes and their corresponding methylases are usually palindromes.

What do palindromes cause?

During DNA replication, the palindromic sequence can result in the formation of a hairpin in the single-stranded lagging strand which can cause replication stalling and a double-strand break.