What kind of syringe is used for mushroom spores?
The 16 gauge needles are the most popular and are recommended if you are making mycelium culture syringes, while the smaller 18 gauge needles are recommended if you are making mushroom spore syringes. We also offer super wide-bore 14 gauge needles used to draw up extra-thick mycelial liquid culture.
Can you see mushroom spores in a syringe?
One syringe has 20 ml of the mushroom spore solution which is enough to inoculate between 6 and 7 liters of substrate. Drop a few milliliters in each corner of the substrate and everything is set. After some days, the growth of white patches of mycelium can be noticed on the spot where the mushroom spores were dropped.
Can you make liquid culture from spore syringe?
Turn one spore syringe into 10 or more! Simply inject 3-5 cc’s of your favorite spores in the culture jar and watch it transform into liquid mycelium. In a few days it will be ready to inject into any substrate jar or spawn bag. 10 ounce Jar of liquid culture solution, pressure sterilized and ready to use.
How many spore syringes do I need?
Typically each jar requires around 0.75-1.5 cc’s of spore solution. Each Spawn Bag requires 3-5 CC’s per bag….Quick reference on how many spore syringes you need:
| Simple Kit or 6 Jars | 1 Spore Syringe (with leftovers) |
|---|---|
| 24 or 30oz 5grain Jars (3) | 1 Spore syringe |
Can you use distilled water to make a spore syringe?
To begin with we need to make sure the water we are using for our spore syringe is completely sterile. I like to use a nice distilled water such as this one. It makes sense to use the best quality water to begin with.
How long can spores survive in a syringe?
What’s the Best Way to Store Mushroom Spores? The best place to store a spore syringe is in your refrigerator. We recommend studying our spores as soon as possible after you receive them, but as we discussed above, you can expect refrigerated syringes to last for up to 30 days and often longer.
How do you use a spore syringe?
Hold the syringe with the cap pointing up. Unscrew the the cap from the spore syringe by turning it counter clockwise. Remove the needle from it’s sterile package (leave the protection cap on the needle) and attach it to the spore syringe. Before use shake the spore syringe for a few seconds.
How do you make oyster mushroom liquid culture?
Firstly, dissolve 20g of LME into 1 litre of water, I tend to this with hot/boiling water as it helps dissolve it better. Fill your jars/jar approximately 80% full with broth. If you have a magnetic stirrer add it now, if not, add a bolt. If you don’t have a bolt, don’t worry you’ll just have to swirl a bit more.
How do you make a liquid culture syringe?
How to use Liquid Culture?
- Sterilize a syringe by filling it, then emptying it with boiling water 3-4 times.
- Wipe the liquid culture jar lid with Alcohol Wipes.
- Flame sterilize the needle until glowing red then insert into hole in the lid of the jar.
- Pull back on the syringe until you have collected some mycelium.