What is RFLP AFLP RAPD?
PRINCIPLE OF AFLP The AFLP technique is based on the principle of selectively amplifying a subset of restriction fragments from a complex mixture of DNA fragments obtained after digestion of genomic DNA with restriction endonucleases. …
What is the difference between AFLP and RFLP?
The key difference between AFLP and RFLP is that AFLP involves selective PCR amplification of the digested DNA while RFLP does not involve selective PCR amplification of the DNA fragments.
What is AFLP analysis?
Amplified fragment length polymorphism (AFLP) analysis is a universal polymerase chain reaction (PCR)-based DNA fingerprinting technique comprising three main stages: (i) digestion of genomic DNA with restriction endonucleases and ligation to double-stranded adaptors (each comprised of two oligonucleotides), thus …
Who had described the technique of AFLP marker?
AFLP-PCR was first described by researcher Pieter Vos and his colleagues in 1995 (Vos et al., 1995). This technique involves five major steps, as described in the following sections.
What is the difference between RAPD and AFLP?
The AFLP technique is more laborious and time con- suming than RAPD methods but is also more reliable, AFLP being able to detect a large number of polymorphic bands in a single lane rather than high levels of polymor- phism at each locus such as is the case for SSR methods.
What is the principle of AFLP marker?
Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest.
Why AFLP is a dominant marker?
AFLP are multilocus markers and their mode of inheritance is dominant. The genotyping technology is rather simple. The main advantages of this system are the relative ease of the genotyping, the relative high number of loci detected in each reaction, and the reliability of the system.
What is AFLP?
Acute fatty liver of pregnancy (AFLP) is a rare, potentially fatal complication that occurs in the third trimester or early postpartum period. Although the exact pathogenesis is unknown, this disease has been linked to an abnormality in fetal fatty acid metabolism.
What is AFLP in DNA fingerprinting?
The AFLP technique is a powerful DNA fingerprinting technology applicable to any organism without the need for prior sequence knowledge. The protocol involves the selective PCR amplification of restriction fragments of a total digest of genomic DNA, typically obtained with a mix of two restriction enzymes.
What is unique component of AFLP?
Amplified fragment length polymorphism (AFLP) is a DNA fingerprinting technique that has features of both RFLP and RAPD (Bensch and Akesson, 2005). AFLP analysis involves digestion of whole genomic DNA with two restriction enzymes.
Which marker combination is suitable for AFLP?
AFLP method is developed by combining the techniques of RFLP and PCR for DNA fingerprinting. Genomic DNA is digested by restriction enzymes and the restricted fragments are preselectively and selectively amplified by different combination of primers.
What is AFLP marker?
Amplified fragment length polymorphism (AFLP) is a PCR-based fingerprinting technique that was first described by Vos et al. The power of AFLP analysis derives from its ability to quickly generate large numbers of marker fragments for any organism, without prior knowledge of genomic sequence.