How do you identify streptococcus bacteria?
Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catalase test, while staphylococci are catalase-positive.
How do you write an unknown bacteria report?
The pronouns “I”, “We” and “They” are not typically used.. For example, instead of writing “I used a TSA agar plate to isolate my unknown,” it is customary to write, “A trypticase soy agar (TSA) plate was used to isolate the unknown.” It is also customary to write in the past tense for most of the report.
What is the presumptive identification of group A streptococci?
In the case of group A streptococci the PYR test is an excellent test for the presumptive identification of group A streptococci. The non-beta hemolytic streptococci (viridans, and non-enterococcal group D) do not grow in 6.5% NaCl broth; but some of the beta-hemolytic strains may grow in the broth.
How do you identify Group A Streptococcus?
To identify S. pyogenes in clinical samples, blood agar plates are screened for the presence of β-hemolytic colonies. The typical appearance of S. pyogenes colonies after 24 hours of incubation at 35-37°C is dome-shaped with a smooth or moist surface and clear margins.
How do you test for streptococcus?
A rapid strep test involves swabbing the throat and running a test on the swab. The test quickly shows if group A strep is causing the illness. If the test is positive, doctors can prescribe antibiotics. If the test is negative, but a doctor still suspects strep throat, then the doctor can take a throat culture swab.
What kind of bacteria is Streptococcus?
Strep is short for Streptococcus, a type of bacteria. There are several types. Two of them cause most of the strep infections in people: group A and group B. Strep throat – a sore, red throat.
How do you write a lab report in microbiology?
The sections required vary from laboratory to laboratory but the standard outline for most lab reports in the biological science include: title, your name, purpose of the experiment, methods, results, discussion and conclusion, references.
What is group A streptococcus bacterial infection?
Group A streptococci are bacteria commonly found in the throat and on the skin. The vast majority of GAS infections are relatively mild illnesses, such as strep throat and impetigo.
What is the ASO test?
Antistreptolysin O (ASO) titer is a blood test to measure antibodies against streptolysin O, a substance produced by group A streptococcus bacteria. Antibodies are proteins our bodies produce when they detect harmful substances, such as bacteria.
Which bacteria are group A strep?
Infection with Streptococcus pyogenes, a beta-hemolytic bacterium that belongs to Lancefield serogroup A, also known as the group A streptococci (GAS), causes a wide variety of diseases in humans.
How do you differentiate between staphylococcus and Streptococcus?
Streptococci are Gram-positive cocci that grow in pairs or chains. They are readily distinguished from staphylococci by their Gram-stain appearance and by a negative catalase test. More than 30 species have been identified.
How are Streptococcus colonies confirmed as enteric unknowns?
Colonies can be confirmed as Streptococcus or Enterococcus by Gram staining. After confirming the morphology of the cells as Gram positive cocci, other biochemical characteristics will allow for a genus and species identification (Table 2). Figure 2: Hemolysis of red blood cells contained in sheep blood agar.
Are there any diseases caused by Streptococcus bacteria?
Many members of the Streptococcus genus are normal flora to the mouth, nose, and throat. The genus Streptococcus is a complex group causing a wide range of diseases such as: rheumatic fever, impetigo, pharyngitis, laryngitis, toxic shock syndrome, scarlet fever, and endocarditis.
How do you figure out the unknowns in microbiology?
The first step to figuring out the unknowns, was to separate the two bacteria. In order to do this, a nutrient agar plate was used. The streak method was used to spread the bacteria across the nutrient agar in hopes of isolating a pure culture of one of the bacteria.
What kind of bacteria was in unknown # 123?
Discussion/Conclusion. The unknown #123 contained two different specimen of bacteria, one being a gram positive bacteria and one being a gram negative bacteria. The first unknown in #123 found to be a gram negative bacteria, was identified as Pseudomonas aeruginosa.