What is GFP purification?
Purification of recombinant GFP from the clarified lysate of N. benthaniana leaves was achieved by using an alcohol/salt aqueous two-phase system (ATPS) and following with a further hydrophobic interaction chromatography (HIC). The purification process takes only ~ 4 h and can recover 34.1% of the protein.
Why are GFP purified?
GFP is purified from the bacterial lysate using hydrophobic interaction chromatography (HIC) columns provided in this kit. Green fluorescent protein is extremely hydrophobic compared to bacterial proteins. Then, simply lowering the salt concentration of the buffer causes GFP to elute from the column in pure form.
What does GFP protein do?
Biologists use GFP as a marker protein. GFP can attach to and mark another protein with fluorescence, enabling scientists to see the presence of the particular protein in an organic structure. Gfp refers to the gene that produces green fluorescent protein.
What is recombinant GFP?
Recombinant GFP (Green Fluorescent Protein) is intended as a convenient positive control for the in vitro synthesis of GFP using the Thermo Scientific 1-Step Human Coupled IVT Kit. More Product Data. • Protocol for in vitro protein expression using mRNA templates.
Is GFP soluble protein?
The green fluorescent protein (GFP) is a powerful reporter protein that allows labeling of specific proteins or entire cells. However, as GFP is a small soluble protein, it easily crosses membranes if cell integrity is disrupted, and GFP signal is lost or diffuse if the specimen is not fixed beforehand.
How much does GFP cost?
Enhanced Green Fluorescent Protein (EGFP)
| SKU-Size | Size | Price |
|---|---|---|
| 4999-100 | 100 μg | $340.00 |
| 4999-1000 | 1 mg | $2,365.00 |
| 4999-5000 | 5 mg | $6,985.00 |
| More Sizes | Get | Quote |
What makes GFP so special?
GFP makes for an excellent tool in many forms of biology due to its ability to form an internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
What is HIC resin?
Macro-Prep HIC Resins are methacrylate-based beads for protein and nucleic acid purification using hydrophobic interactions. Methyl and t-butyl supports are available for purifying compounds that have weak or strong hydrophobic regions.
What are the patents for fruit fluorescent proteins?
The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511 and 7,393,923. Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed-Monomer) can be used as a standard for protein gels, Western blots, and fluorometry.
Which is a purified recombinant green fluorescent protein?
Purified, recombinant fluorescent proteins—GFP, DsRed. Affinity purified recombinant Aequorea coerulescens green fluorescent protein (rAcGFP1) can be used as a standard for protein gels, Western blots, and fluorometry. It can also be used as a control for expression studies and for microinjection into cells and tissues.
How is racgfp1 used in microinjection studies?
It can also be used as a control for expression studies and for microinjection into cells and tissues. At its N-terminus, rAcGFP1 contains a 6xHN epitope tag, used for purification of the protein by immobilized metal affinity chromatography (IMAC) with our TALON Resin.
Are there any antibodies that can detect GFP?
The Full-Length GFP Polyclonal Antibody detects AcGFP1, wild type GFP, and GFPuv. Based on their homology to GFP, we also expect this antibody to detect EGFP, EYFP, and ECFP.