What are the different types of embedding medium?

What are the different types of embedding medium?

Embedding Media, Paraffin, Paramat, Paraplast, Peel Away Paraffin, Tissue Freezing Medium, Cryogenic-Gel, O.C.T. Compound, Polyfin, Polyester Wax.

What is the embedding medium?

The most used embedding medium is paraffin. After cooling, it is in the presence of a paraffin block, hard, inside which the removed part is included. In some cases, other inclusion media (celloidin, plastic resins, etc.) are used.

Which of the following embedding medium is used when frozen sections are required for cryo sectioning?

The frozen section refers to the process where there is rapid tissue section cooled with a cryostat to provide an immediate report of the tissue sample. The cryostat freezes the tissue allowing it to be cut for a microscopic section. The conversion of water into ice acts as the embedding media for cutting the tissue.

What is the most commonly used embedding medium?

Paraffin wax is the most commonly used embedding medium.

Why is xylene used as a clearing agent?

Xylene is a chemical commonly used in the histology lab as a clearing agent. Clearing agents are used to make the slides easier to read, by making the tissue transparent, or clear. Xylene is used because wax is not soluble in water. Alcohol replaces the water in the tissue.

Is xylene an embed medium?

The most widely used embedding medium for biological applications is paraffin wax, or ‘paraffin. ‘ Paraffin wax is insoluble in water but dissolves in toluene and xylene and melts at 46–68 °C, making it an ideal medium for embedding tissues after they have been dehydrated.

Is commonly used embedding medium?

What is the size of ideal section in frozen sectioning?

This is a mandatory step when using a warm chuck or using the large 30 mm well. Optimal freezing conditions refer to completely cooled well bars and chucks stored in a cryostat at -24 °C. Optimal freezing conditions are as follows: 18 mm well, 20 sec; 24 mm well, 35 sec; 30 mm well, 60 sec.

What can you use instead of xylene?

Carrot oil, Olive oil, Pine oil, Rose oil, are not only bio friendly and economical but can also be used as clearing agent instead of xylene.

Which is the best cryo embedding compound for frozen sections?

PELCO ® Cryo-Embedding Compound is a water soluble glycol medium for use in embedding for frozen sections. This low viscosity compound assures reliable freezing and smooth sections. PELCO ® Cryo-Embedding Compound is a fully compatible, cost effective alternative to Product #27050, Tissue Tek ® O.C.T. Compound.

Which is the best cryo embedding media for Mohs?

PolarStat Plus™ is an exceptional cryo embedding media that works well with an anti-roll plate or may be led off the blade with a brush. It is especially well-suited for Mohs cryostat sectioning. Now available in 4 optimal colors to accompany the traditional white formulation. This formulation freezes at -10°C.

Which is the best cryo tray to use?

The SEAL’N FREEZE ® CRYOTRAY ® is a compact system of plastic cryo embedding molds with attached sealable lids. This tray system allows for batch processing of tissue samples in perfect uniform blocks that are ideal for cryo sectioning and storage.

How are embedding wells used in cryosectioning?

Embedding is performed in wells machined into the face of one-inch thick stainless steel bars ( Figure 1A ). When cooled to cryostat temperature, these rather substantial bars provide a powerful heat sink for rapid freezing. Wells are machined with beveled walls, rounded edges, and polished surfaces for ease of release.