What does the Genesys 20 measure?

What does the Genesys 20 measure?

The Genesys 20 – Setting absorbance and % transmittance In addition to taking absorbance and %transmittance measurements, you can also set specific values for the absorbance or %transmittance on the display. Press the and keys to set the absorbance or %transmittance value on the display.

How long must the instrument warm up before it should be used?

It must warm up for 30 minutes before use. Set the instrument to the proper wavelength by turning the knob located on the right hand surface of the spectrophotometer.

How does a spec 20 work?

The Spectronic 20, often called the Spec 20, is a spectrophotometer. A spectrophotometer measures the intensity of a light beam before and after it passes through a sample and compares these two intensities. The Spec 20 reports two types of measurements: percent transmittance and absorbance.

What is the purpose of zero and 100 t adjustment?

This adjustment tells the instrument what signal corresponds to no light being absorbed.

How do you use a UV spectrophotometer?

Procedure

1. Turn on the UV-Vis spectrometer and allow the lamps to warm up for an appropriate period of time (around 20 min) to stabilize them.
2. Fill a cuvette with the solvent for the sample and make sure the outside is clean.
3. Place the cuvette in the spectrometer.
4. Take a reading for the blank.

How do you use a digital spectrophotometer?

Plug in and turn on the spectrophotometer. Allow it to warm up for 15 minutes. Press the Percent T/A selector to select Percent Transmittance or Percent Absorbance mode. Locate the wavelength dial beside the sample chamber and set it to the desired wavelength.

Why does a spectrophotometer need to warm up?

The visible spectrometer most commonly encountered in the general chemistry laboratory is the Spectronic 20 (manufactured by Milton Roy). The spectrometer should be allowed to warm up for at least fifteen minutes to stabilize the source and detector. 2.

How does the spec 20 work?

The Spectronic 20, often called the Spec 20, is a spectrophotometer. A spectrophotometer measures the intensity of a light beam before and after it passes through a sample and compares these two intensities. You must select a wavlength and calibrate the instrument at that wavelength before making any measurements.

Why is water used as a blank?

Water is used because it is transparent. The blank is used so the absorbance from it can be added to any light that is absorbed or reflected from the sample. The blank is used so the absorbance from it can be subtracted from any light that is absorbed or reflected from the sample.

How do you zero a Spectrophotometer?

This allows you to set a “0” so that your absorbance readings will have a basis to be compared to. Zero (blank) the SmartSpec 3000 by placing a cuvette containing only media (NO BACTERIA) into the cuvette chamber and pressing Read Blank. You can re-zero the instrument at any time during use.

What kind of spectrometer is the Genesys 20?

Spectroscopy on the Genesys 20®. BACK. The Spectronic 20 (Spec20) is the most common type of spectrophotometer used in labs today, and is the one you will use in your CHM1046 labs. The spectrophotometer is an instrument that measures the amount of light that passes through a medium at a specific wavelength.

How does the Spectronic 20 Genesys control pad work?

The Spectronic 20 Genesys control pad. Decreases the measurement wavelength. Increases the measurement wavelength. Sets the absorbance to 0 or the %transmittance to 100% at the current wavelength. Toggles between Absorbance, %Transmittance and Concentration measurement modes. Allows the user to set various default parameters.

Who is the manufacturer of the Genesys 20?

The Genesys 20 instrument can only be internally serviced and calibrated by the manufacturer (Thermo Fisher Scientific). GEX recommends that the calibration performed by the manufacturer of the Genesys 20 be verification tested periodically to ensure that the instrument is operating within specified limits.

Is the Thermo Scientific Genesys 30 still available?

Please call customer service for assistance: 1-800-766-7000. This product has been discontinued. Please check out the newest visible spectrophotometer, the Thermo Scientific™ GENESYS™ 30. Multiple purpose capabilities allow you to measure a wide range of analytes.