What is the LD50 of ethidium bromide?

What is the LD50 of ethidium bromide?

1503 mg/kg.
Ethidium Bromide:, LC50 (inhalation, rat): 0.0118 – 0.1340 mg/L/6H. LD50 (oral,rat): 1503 mg/kg., By mists determined to be toxic based on: LC50 (inhalation rat) 0.0472mg/L/1H * 100 (dilution rate)=approximately 4.72mg/L/1H.

What is ethidium bromide chemical hazard rating?

Hazard statement(s) H302 Harmful if swallowed. H330 Fatal if inhaled. H341 Suspected of causing genetic defects.

Is ethidium bromide toxic?

Because ethidium bromide can bind with DNA, it is highly toxic as a mutagen. It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found.

What happens if ethidium bromide is exposed to skin?

EtBr is a potent mutagen (can cause genetic damage), and moderately toxic after an acute exposure. EtBr can be absorbed through skin, so it is important to avoid any direct contact with the chemical. The powder form is considered an irritant to the upper respiratory tract, eyes, and skin.

Is EtBr hydrophobic?

Ethidium bromide is known as an intercalate agent as it inserts itself between the stacked bases of DNA. The ring structure of ethidium bromide resembles the rings of DNA bases and it is also hydrophobic.

What are the sizes of the three DNA bands?

Since all DNA should migrate in the same way (i.e., assuming the same conformation (shape) of the DNA and uniform charge), we can eyeball the size of the unknown band. It looks like it’s between 3.5 kb and 4 kb. This is just a rough guess, though.

Is hydrogen peroxide a hazmat?

This chemical is considered hazardous by the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200).

Is EtBr radioactive?

Ethidium bromide (EtBr) is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and in other methods of nucleic acid separation.

How do you dissolve EtBr?

Ethidium Bromide Solution Preparation and Recipe

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 10 g of Ethidium bromide to the solution.
3. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.

What is the function of EtBr?

The EtBr works as a color agent that gives color to DNA. EtBr works as a separating agent in agarose gel electrophoresis. EtBr intercalates between DNA base pairs and emits fluorescence under UV light.

What are the health effects of ethidium bromide?

It may potentially cause carcinogenic or teratogenic effects, although no scientific evidence showing either health effect has been found. Exposure routes of ethidium bromide are inhalation, ingestion, and skin absorption. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes.

Is it safe to give ethidium bromide to cattle?

Ethidium Bromide: A Reality Check. For this purpose, ethidium bromide is administered via subcutaneous or intramuscular injection with no reported increase in incidence of tumor formation or birth defects in the treated cattle. This suggests that ethidium bromide is far less genotoxic to animal systems than is presumed from the in vitro data.

What’s the best way to dispose of ethidium bromide?

The method of choice for ethidium bromide disposal at the moment seems to be absorption onto charcoal followed by incineration. You have a 1 microgram/litre solution of ethidium bromide, a concentration that appears to be of low toxicity according to the data from cattle.

Do you have to wash your hands with ethidium bromide?

Always wear gloves when working with ethidium bromide and ethidium-stained gels. Also, while wearing gloves after handling ethidium bromide, be careful not to touch and thereby contaminate other surfaces. Be sure to wash hands with soap and warm water after working with ethidium bromide.