What is thick and thin blood film?
A thick blood smear is a drop of blood on a glass slide. A thin blood smear is a drop of blood that is spread across a large area of the slide.
What is the principle of thick blood film?
Thick smears consist of a thick layer of dehemoglobinized (lysed) red blood cells (RBCs). The blood elements (including parasites, if any) are more concentrated (app. 30×) than in an equal area of a thin smear. Thus, thick smears allow a more efficient detection of parasites (increased sensitivity).
What is the difference between thick film and thin film?
Thin film has a thickness in the order of 0.1 um (micrometer) or smaller, while thick film is thousands times thicker. However, the main difference is the method used to apply the resistive film onto the substrate. Thin film is more accurate, has a better temperature coefficient and is more stable.
Which of the following is a key characteristic of the thick blood film?
Which of the following is a key characteristic of the thick blood film? RBCs must be laked during or before Giemsa staining. When using fecal fixatives, it is mandatory to thoroughly mix the stool specimen with the fixative.
What is the difference between blood smear and blood film?
Left smear is unstained, right smear is stained with Wright-Giemsa stain. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically.
What is difference between thick blood film and thin blood film?
What is the importance of preparing a good blood film?
Inadequately prepared smear can present different artifacts and lead to errors in the differential count. Blood films should be made immediately after collection of the blood, because cell morphology deteriorates rapidly after sample collection.
When is the best time to get malarial smear preparation?
Malaria: BLOOD SHOULD BE COLLECTED IMMEDIATELY UPON SUSPICION OF MALARIA, although the optimum time is about midway between chills to ensure obtaining stages on which species identifications can be made. Since single blood smears may not reveal organisms, successive smears at 6, 12 or 24 hours are sometimes necessary.
Why is thick blood film not fixed?
Thick films should not be fixed with methanol (or direct heat). During the aqueous staining procedure the unfixed RBC lyse, leaving the remains of WBC and any malaria parasites present behind. If thick films are fixed before staining they will be too dense to examine.
What is the principle of Giemsa stain?
PRINCIPLE: The “neutral” dyes combining the basic dye methylene blue and the acid dye eosin, give a wide color range when staining. The pH of the staining solution is critical and ideally should be adjusted for different fixatives.
What does blood film test indicate?
A blood film examination allows the evaluation of white blood cells (WBCs, leucocytes), red blood cells (RBCs, erythrocytes), and platelets (thrombocytes). These cell populations are produced and mature in the bone marrow and are eventually released into the bloodstream as needed.
What is the purpose of thick blood smear?
A thick blood smear is a drop of blood on a glass slide. Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger sample of blood. (Often there are few parasites in the blood at the time the test is done.)
What’s the difference between a thick and thin blood smear?
Thick blood smears are most useful for detecting the presence of parasites. Thin blood smears helps to discover which species of parasite is causing the infection. A thick blood smear is a drop of blood on a glass slide. A thin blood smear is a drop of blood that is spread across a large area of the slide.
Why is it important to know about thick blood?
Thick blood on its own does not cause health problems, but certain medical conditions that cause blood to become thick can severely affect a person’s life. People who have thick blood are at greater risk of blood clots in the arteries and veins. Anyone who thinks they are experiencing a blood clot should seek medical attention immediately.
How is the morphology of a blood film recorded?
After staining, the monolayer is viewed under a microscope using magnification up to 1000x. Individual cells are examined and their morphology is characterized and recorded. The left image shows a microscopic view of a normal adult blood film, while the right image shows a blood film from a patient with chronic myeloid leukemia.
Which is more sensitive thick film or thin film?
Thick films allow the microscopist to screen a larger volume of blood and are about eleven times more sensitive than the thin film, so picking up low levels of infection is easier on the thick film, but the appearance of the parasite is much more distorted and therefore distinguishing between the different species can be much more difficult.