What is the molecular weight of GFP?
27 kD
The GFP cDNA consists of 730 bp, which encode a 238 amino acid protein with a molecular weight of 27 kD (2). Wild-type GFP emits a vibrant green fluorescence upon exposure to blue light (450-490 nm).
What is the number of amino acids and the molecular weight of the in the GFP protein and Dsred monomers?
The native Aequorea victoria GFP is composed of a single-domain protein with 238 amino acids and a molecular weight of approximately 27 kDa.
What is the isoelectric point of GFP?
U57607) is a protein composed of 293 amino acid residues (32.7 kDa) that has an isoelectric point of 6.2 and exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
What size in units of kDa do you predict for the Hexahistidine tagged GFP fusion protein?
Intact mass analysis of Ni2+-NTA eluates of His-tagged GFP (top) reveals the expected molecular mass of 28.9 kDa. His-tagged GFP-perlucin (bottom) yields two major peaks at 29.8 kDa and 30.3 kDa corresponding to truncated protein variants.
How many AA is GFP?
Green fluorescent protein (GFP) is a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light. The protein has 238 amino acids, three of them (Numbers 65 to 67) form a structure that emits visible green fluorescent light.
How is GFP tagging done?
GFP-tagging is a way of preparing a sample for fluorescence microscopy by using the GFP as a fluorescent protein reporter. This is done by cloning the GFP in frame with the target protein at either the N- or C-terminus of the amino acid chain.
What is the molecular weight of BSA in g mol?
66430.3 g/mol
Based on the amino acid sequence, the molecular weight of bovine serum albumin is 66430.3 daltons, which is equivalent to 66430.3 g/mol.
How do you find the molecular weight of BSA?
The mass of BSA is ~66430 Da. Use the unit conversion of 1 Da = ~1 g/mol. Since 0.1 mg/ml is equivalent to 0.1 g/L, then the molar concentration of 0.1 mg/ml is calculated by (0.1 g/L) / (66430 g/mol) or ~1.5 E-6 mol/L.
Where does the green fluorescent protein GFP come from?
Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm.
How many amino acids are in the GFP protein?
The protein has 238 amino acids, three of them (Numbers 65 to 67) form a structure that emits visible green fluorescent light. In the jellyfish, GFP interacts with another protein, called aequorin, which emits blue light when added with calcium.
How is GFP used to analyze colocalization of proteins?
GFP can be used to analyse the colocalization of proteins. This is achieved by “splitting” the protein into two fragments which are able to self-assemble, and then fusing each of these to the two proteins of interest. Alone, these incomplete GFP fragments are unable to fluoresce.
Can a GFP molecule fluoresce different colors?
They are engineering GFP molecules that fluoresce different colors. Scientists can now make blue fluorescent proteins, and yellow fluorescent proteins, and a host of others. The trick is to make small mutations that change the stability of the chromophore.