What magnification do you need to see onion root tip?
Set up your microscope, place the onion root slide on the stage and focus on low (40x) power. move your slide so that your field of view is centered on the root tip. Focus at 100x and re center so that you are focused on the more ‘square’ meristem cells.
What is the mitotic index of onion root tip?
The mitotic index of the onion root tip 1 and 2 was calculated and recorded in table 3. The mitotic index was predicted to be huge due to observing the root region of division of the root but was actually under 50% with a 26% value for both root tips….Genetics.
| Phase | Time (min) |
|---|---|
| G3 | 884 x .3 = 265.2 |
Why do we observe an Allium root tip to observe the different stages of mitosis?
The root tip of a plant contains actively dividing cells, making root tips excellent observation points for mitosis. Each plant cell is surrounded by a cell wall, making the identification of cells easy. Also, the cells near the root tip are highly organized into concentric layers, making them easy to see and count.
At what magnification can you see mitosis?
Focusing the microscope with 40x objective should give you a close enough view of the chromosomes to find each phase. You will also look for spindle fibers which are attached to each chromosome and are used by the cell to separate the chromosomes and move them to each pole.
Why is Allium good for studying mitosis?
Why is a sample from the roots of Allium spp. a good specimen for studying mitosis? Cells are actively growing so it is easy to find cells in various stages of mitosis.
Why we use HCL in onion root tip?
1. The root tip is heated with acid to break up the tissues into individual cells. The cellulose walls of plant cells are held together by pectins such as calcium pectate. Treatment with hydrochloric acid breaks this down.
What is Allium root tip?
Allium root tip: Examine the square cells just inside the root cap. This is the root meristem (embryonic tissue) where mitosis is occurring. Farther up the root is the elongation zone, where cells are long rectangles; these cells are not undergoing mitosis.
What happens at the end of anaphase?
At the conclusion of anaphase, each end of the cell has an identical and complete set of 46 chromosomes or 23 pairs of homologous chromosomes; they are still diploid. Telophase: Telephase begins once the chromosomes have completed separating and reached the opposite poles of the cell.
How many chromosomes does the Allium root tip have?
16
Common onion (Allium cepa L.) has eight pairs of relatively large chromosomes (2n = 16) that allows for the easy detection of CAs.