What is the function of eEF2?
Molecular Mechanisms of Memory Eukaryotic elongation factor 2 (eEF2) is a GTP-binding protein that mediates the translocation of peptidyl-tRNA from the A site to the P site on the ribosome (Moldave, 1985).
What is EF2 in biology?
EF2 (or EF-G) is responsible for the translocation of the peptidyl-tRNA from the A-site to the P-site (peptidyl-tRNA site) of the ribosome, thereby freeing the A-site for the next aminoacyl-tRNA to bind.
Where does eEF2 bind?
Binding of eEF2 stabilizes the swiveled head conformation of the ribosome and the ap/P state of tRNA through an interaction of eEF2 domain IV with the peptidyl-tRNA in the P-site and the decoding center. If the E-site contains cognate deacylated tRNA, then it adopts the pe/E-state.
Which toxin inhibit elongation factor 2?
Eukaryotic elongation factor 2 is the target of a class of bacterial mono-ADP-ribosyltransferase toxins which include the prototype, DT, exotoxin A from P. aeruginosa, and cholix toxin from V. cholera. Exposure of eukaryotic cells to these toxins leads to inhibition of protein synthesis and cell growth.
What is translocation in protein synthesis?
In the elongation cycle of translation, translocation is the process that advances the mRNA–tRNA moiety on the ribosome, to allow the next codon to move into the decoding center.
Which elongation factor is responsible for the binding of each aminoacyl tRNA to the ribosome?
Elongation factors (Table 1) in bacteria (e.g., EF-Tu also known as EF1A) and in eukaryotes (e.g., the eukaryotic Elongation Factor 1 Complex [eEF1A]) all have the same primary and critical function to shuttle aminoacylated tRNAs to the ribosome during protein translation.
Is EF2 AG protein?
As a GTPase, EF-G catalyzes the movement (translocation) of transfer RNA (tRNA) and messenger RNA (mRNA) through the ribosome….EF-G.
| EFG/EF2, domain IV | |
|---|---|
| Identifiers | |
| SMART | SM00889 |
| CDD | cd01434 |
| showAvailable protein structures: |
What is elongation biochemistry?
Translation elongation factors are the workhorses of protein synthesis on the ribosome. They assist in elongating the nascent polypeptide chain by one amino acid at a time. The general biochemical outline of the translation elongation cycle is well preserved in all biological kingdoms.
What does EF-G do in translation?
EFG is the translocase of translation, it catalyzes the movement of the peptidyl-tRNA from the A-site to the P-site and deacetylated tRNA from the P-site to the E-site of the ribosome [9], [10].
What is ADP ribosylation EF2?
ADP-ribosylated EF2 (ADPR•EF2) is inactive in catalyzing the translocation of peptidyl-tRNAs on the ribosome, thus preventing nascent protein synthesis. These findings suggest that inhibition of nascent protein syntheses may result from ADPR•EF2 abolishing the upstream event(s) before translocation.
Which type of exotoxin is diphtheria toxin?
Diphtheria toxin is an exotoxin secreted by Corynebacterium, the pathogenic bacterium that causes diphtheria. The toxin gene is encoded by a prophage called corynephage β….Diphtheria toxin.
| Diphtheria toxin, C domain | |
|---|---|
| Identifiers | |
| TCDB | 1.C.7 |
| showAvailable protein structures: |
Where does translocation occur?
phloem
Translocation occurs within a series of cells known as the phloem pathway, or phloem transport system, with phloem being the principal food-conducting tissue in vascular plants. Nutrients are translocated in the phloem as solutes in a solution called phloem sap.
What is the function of the EEF2 gene?
Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. It is the archaeal and eukaryotic counterpart of bacterial EF-G. This gene encodes a member of the GTP -binding translation elongation factor family. This protein is an essential factor for protein synthesis.
How is the EF2 gene inactivated in the ribosome?
It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008] An internal error has occurred that prevents Sequence Viewer from displaying.
How many amino acids are in the EF2 protein?
Rapp et al. (1989) reported the complete sequence of the predicted 858-amino acid EF2 protein. Sequence comparisons revealed that the hamster, rat, and human EF2 protein sequences differ in only 8 positions.
Which is member of the E2F family of transcription factors?
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.