What is the composition of Giemsa stain?
Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue. The thin film is fixed with methanol.
What is giemsa powder?
Giemsa dye is a blend of several different dyes – Azure, Eosin, and Methylene Blue dyes. Giemsa dye is used for routine staining of blood smears and blood marrow samples. It is also used for identifying microorganisms, chromosomatic aberrations, visualization of chromosomes and mastocytes.
How do you mix Giemsa stain?
Recipe
- Dissolve 3.8g of Giemsa powder into 250ml of methanol.
- Heat the solution from step 1 to ~60oC.
- Slowly add in 250ml of glycerin to the solution from step 2.
- Filter the solution from step 3.
- The solution needs to stand a period of time prior to use.
What are the components of the dye in Wright Giemsa stain solution?
Wright Giemsa Stain is a mixture of Azure B, Methylene blue, and Eosin dye. Azure B and eosin are acidic dyes while methylene blue is the basic dye.
How do you make a 10% Giemsa stain?
Make up a 10% Giemsa solution with distilled/deionized water buffered to pH 7.2. If only one slide is to be stained, you will require about 3 ml of prepared stain. Allow 3 drops of stock Giemsa solution (from the Pasteur pipette) to each millilitre of buffered water to give a 10% solution.
Which structures does Giemsa stain quizlet?
It can be used for histopathological diagnosis of malaria and some other spirochete and protozoan blood parasites. Giemsa/Wright’s stain is a classic blood film stain for: peripheral blood smears and bone marrow specimens. platelets show a light pale pink.
How do you make a 3% Giemsa stain?
Step 3 Prepare a 3% solution of Giemsa stain by adding 3 ml of Giemsa stock solution to 97 ml of buffered water.
What is the purpose of glycerol in Giemsa stain?
In 1904 Giemsa published an essay on the staining procedure for flagellates, blood cells, and bacteria. Giemsa improved the Romanowsky stain (Eosin Y and Methylene Blue) by stabilizing this dye solution with glycerol. This allowed for reproducible staining of cells for microscopy purposes.
How do you make 3% of Giemsa stain?
Step 3 Prepare a 3% solution of Giemsa stain by adding 3 ml of Giemsa stock solution to 97 ml of buffered water. Step 4 Pour the stain gently into the trough until the slides are totally covered. Avoid pouring the stain directly on to the thick films. Step 5 Leave the slides in the stain for 30-45 minutes.
How do you make a 5% Giemsa stain solution?
Popular Answers (1)
- Dissolve 3.8g of Giemsa powder into 250ml of methanol.
- Heat the solution from step 1 to ~60oC.
- Slowly add in 250ml of glycerin to the solution from step 2.
- Filter the solution from step 3.
- The solution needs to stand a period of time prior to use.
What does Giemsa stain bind to?
Giemsa stain is specific for the phosphate groups of DNA. It attaches itself to regions of DNA with high amounts of adenine-thymine bonding. Giemsa stain is used in Giemsa banding (G-banding), to stain chromosomes and it is often used to create a diagrammatic representation of chromosomes (idiogram).
What is the pH of Giemsa stain?
pH 6.8
Such stains as Leishman, Wright’s Blood Stain and May Granwald/Giemsa are commonly used in hematology laboratories in the UK and all of these are used at a slightly acid pH (pH 6.8).
What kind of dye is used in special stain-Giemsa?
Special Stain – Giemsa. The basic principle underlying the stain is that a basophilic or basic dye, methylene blue, is combined with eosinophilic acidic dyes, eosin, azure A and azure B – to create “neutral dyes” that demonstrate a wide variety of colours when used to stain haematopoietic cell nuclei and platelets.
How is methanol used as a fixative in Giemsa stain?
Methanol act as a fixative as well as the cellular stain. The fixative does not allow any further change in the cells and makes them adhere to the glass slide. Giemsa stain can be prepared in house using Giemsa stain powder or can be commercially obtained. The basic ingredients of both are the same; however, dilutions can be made depending on use.
How to prepare Giemsa stain for thin blood smear?
Because different procedures are used for the study of blood cells or detection of parasites in blood smears (thin blood smear and thick blood smear). For thin smear, prepare a 1:20 ratio of giemsa stain by mixing 2 ml of stock solution of Giemsa stain to 40 ml of phosphate buffer solution. You may use Distilled water instead of buffer.
What kind of stain is the Romanowsky stain?
Romanowsky stain is a mixture of mixture of oxidized methylene blue, azure, and Eosin Y. This stain is applied on a air-dried slide which is post-fixed with methanol. Methylene blue has a high affinity for acidic components of the cell such as Nucleus, becasue it is a basic dye.