What wavelength does blue dextran absorb at?
The elution of Blue Dextran can be conveniently monitored using absorbance at wavelengths of 254, 280 or 620 nm.
Why is blue dextran used?
Blue dextran has long been used as a void volume marker in chromatography. Blue dextran can also be used as a molecular marker.
What Colour is dextran blue?
Blue Dextran is a high-molecular-weight glucose polymer (original mol wt 2 x 10(6) g/mol) containing covalently bonded Reactive Blue 2 dye (approximately mmol/g dextran). This blue dye is known for its high binding affinity to a wide variety of proteins, with a particularly high affinity for serum albumin.
What is the size of blue dextran?
∼2,000 kDa
The Vo of a given column is based on the volume of effluent required for the elution of a large molecule such as blue dextran (molecular mass of ∼2,000 kDa, Catalog Number D4772).
What is the molecular weight of dextran?
Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.
Does blue dextran elute first?
From the results of this experiment, it was indicated that blue dextran (blue band) had a greater molecular weight than DNP-glycine (yellow band), as it was eluted first.
Why did the blue dextran and the DNP-glycine separate in the column?
Blue dextran and DNP-glycine are separated on the basis of molecular weight by gel permeation (Biogel P100) chromatography. Adherent macromolecules are subsequently eluted from the columns. The presence of each type of protein is verified on the basis of color or, in the case of albumin, absorbance at 280 nm.
Is dextran a protein?
22.2. Dextran is a complex polysaccharide derived from bacteria and it consists of glucose subunits. Dextran, not only seems to possess antithrombotic properties, but it also forms scaffolds that are resistant to protein and cell adhesion.
What is meant by dextran?
Dextran is a complex branched glucan (polysaccharide derived from the condensation of glucose), originally derived from wine. IUPAC defines dextrans as “Branched poly-α-d-glucosides of microbial origin having glycosidic bonds predominantly C-1 → C-6”. Dextran chains are of varying lengths (from 3 to 2000 kilodaltons).
What is blue dextran molecular weight?
The Vo of a given column is based on the volume of effluent required for the elution of a large molecule such as blue dextran (molecular mass of ∼2,000 kDa, Catalog Number D4772).
Which of the following is used in the precipitation of dextran?
Which of the following is used in the precipitation of dextrans? Explanation: Organic solvents like methanol, ethanol can be used in the precipitation of proteins.
How is Blue dextran used in gel filtration?
Blue dextran is a soluble dextran derivative consisting of the triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran ( Sigma-Aldrich, 1997 ). It was developed for use as a visible marker for void volume in gel filtration chromatography columns ( Dean and Watson, 1979, Subramanian, 1984 ).
When to use Blue dextran void volume marker?
Void volume marker for gel filtration columns. Pricing and availability is not currently available. Looking for similar products? Visit Product Comparison Guide Blue dextran is used in affinity chromatography, gel filtration chromatography, protein chromatography and molecular weight markers.
How is a blue dye attached to a dextran molecule?
Blue dextran is a collection of molecules of dextran to which a blue dye is covalently attached. Most of the molecules of dextran are so large and so irregular in shape that they cannot penetrate the beads at all, but there may be some of the blue dextran molecules that are smaller and elute at a volume greater than the void volume.
How big is the running buffer for Blue dextran?
Size markers: Blue Dextran (2 mDa, GE Healthcare), Bovine Thyroglobulin (669 kDa, GE Healthcare), Conalbumin (75 kDa, GE Healthcare). Gel filtration running buffer: 100 m M Hepes, pH 7.9, 150 m M NaCl, 1.5 m M MgCl 2. 1. Equilibrate gel filtration column (e.g., HiPrep 16/60 Sephacryl S-400 High Resolution) with gel filtration running buffer. 2.