What is Ly6G?
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow.
Is Ly6G a surface marker?
About InVivoMAb anti-mouse Ly6G/Ly6C (Gr-1) Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations. The RB6-8C5 antibody has been shown to inhibit the binding of the 1A8 antibody.
Do neutrophils express Ly6C?
Expression of Ly6C is found on neutrophils, monocytes, DCs, and subsets of CD4+ T cells and CD8+ T cells (Table 2). Prominent expression is seen on myeloid cells in the bone marrow as they terminally differentiate into neutrophils and monocytes [48].
How do mice deplete neutrophils?
Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies.
What cell types express Ly6G?
Ly6G – a marker for monocytes, granulocytes and neutrophils. Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow.
Is Ly6G intracellular?
Intracellular Ly6G staining overcomes surface unavailability A known yet often overruled issue in cell depletion strategies is that of antigen masking, whereby an antibody used in vivo will prevent the binding of the same antibody if used to detect the target cells.
Do eosinophils express Ly6G?
1 Based on flow cytometry analysis of blood samples from mouse models, eosinophils have been assumed to express very little or no Ly6G, and Abs to this Ag have been used primarily as universal markers for neutrophils. There was also a mouse strain difference in Ly6G expression in eosinophils.
What is Gr1 marker?
Gr1 is an antibody against Ly6G (granulocyte marker) and Ly6C (macrophage marker) – both are of the Ly6 family of GPI anchor proteins.
What is the function of Ly6C?
Ly6C is a marker of macrophage subsets and support a model of selective recruitment of Ly6C(high) bone marrow monocytes to the kidney that differentiate into three populations of kidney macrophages, including a profibrotic Ly6C(low) population.
What happens if neutrophils are high?
Having a high percentage of neutrophils in your blood is called neutrophilia. This is a sign that your body has an infection. Neutrophilia can point to a number of underlying conditions and factors, including: infection, most likely bacterial.
What does Ly6G stain for?
Ly6G is considered to be the most reliable marker to identify neutrophils. However, you have to keep in mind that Ly6G is also expressed in monocytes and granulocytes.
Do mast cells express Ly6G?
Histamine is a biogenic amine that is chiefly produced in mast cells and basophils and elicits an allergic response upon stimulation. Notably, a CD11b+Ly6G+Ly6Clow myeloid cell population accumulated in the lung during sepsis, and most of these cells expressed high levels of GFP and indeed contain histamine.