What diseases does microarray test for?
A microarray is the recommended first line genetic test for developmental delay (DD), intellectual disability (ID) and autism spectrum disorders (ASD)*. CMA however, does not identify fragile X syndrome (FXS), a common cause of intellectual disability.
How does a microarray test work?
The principle behind microarrays is that complementary sequences will bind to each other. The unknown DNA molecules are cut into fragments by restriction endonucleases; fluorescent markers are attached to these DNA fragments. These are then allowed to react with probes of the DNA chip.
How accurate is microarray testing?
Overall, microarray was successful in 98.8% of cases (4340 of 4391). The microarray analysis was performed on uncultured samples for 3860 (87.9%) of the 4391 participants.
How much does a microarray test cost?
These tests are commercially available for $1500-$2000. However, like all medical testing, discounted costs are often arranged between a hospital and reference laboratory or insurance carrier and reference laboratory, which may bring the actual cost of the test quite lower.
How accurate is microarray?
We found that only approximately 70% of the genes spotted on the microarray matched the correct sequence of the clones. Other groups reported similar observations.
What is application of microarray?
The current scope of microarray applications includes sequencing by hybridization, resequencing, mutation detection, assessment of gene copy number, comparative genome hybridization, drug discovery, expression analysis, and immunoassay (protein microarrays).
When would you use a microarray?
Microarrays can also be used to study the extent to which certain genes are turned on or off in cells and tissues. In this case, instead of isolating DNA from the samples, RNA (which is a transcript of the DNA) is isolated and measured. Today, DNA microarrays are used in clinical diagnostic tests for some diseases.
How is a microarray a form of biotechnology?
Microarray technology is a developing technology used to study the expression of many genes at once. It involves placing thousands of gene sequences in known locations on a glass slide called a gene chip. Complementary base pairing between the sample and the gene sequences on the chip produces light that is measured.
What does a normal microarray mean?
Normal: There are no missing or extra pieces of chromosomes, and no large parts of the chromosomes are the same. Likely pathogenic: There is a missing or extra piece of chromosome that may cause health or learning problems.