What is the function of FITC?
Fluorescein-5-isothiocyanate (FITC) is a fluorescence dye and belongs to the xanthene dyes. FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides.
How does FITC bind to protein?
Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins vi a the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
What is FITC antibody?
FITC (fluorescein isothiocyanate) is a fluorochrome dye widely used as an antibody or other probe marker. FITC absorbs ultraviolet or blue light, exciting molecules which then emit a visible yellow-green light. When the excitation light is removed, the emission light ceases.
What is FITC IHC?
Rb SABC-FITC IHC Detection Kit Description This system involves the sequential incubation of the sample with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, fluorescein-conjugated streptavidin, and chromogen substrate.
What is the meaning of FITC?
Financial Institutions Training Centre (FITC)
What does FITC a mean flow cytometry?
Fluorescein isothiocyanate
Fluorescein isothiocyanate (FITC), for example, will absorb light from 400-530 nm but absorbs most efficiently at its peak or excitation maximum of 490 nm wavelength.
What is FITC conjugated?
FITC is a small organic molecule, and is typically conjugated to proteins via primary amines (i.e., lysines). Usually, between 3 and 6 FITC molecules are conjugated to each antibody; higher conjugations can result in solubility problems as well as internal quenching (and reduced brightness).
What is the molecular weight of FITC?
389.382 g/mol
Fluorescein isothiocyanate/Molar mass
What is Fluorochrome in flow cytometry?
Fluorescent dyes (or fluorochromes) are commonly used as detection reagents in various applications such as cellular imaging and flow cytometry. Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength.
Is FITC same as 488?
High-performance alternatives to fluorescein The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.
What is FITC conjugate?
How are antibodies conjugated?
Antibody conjugation, also known as antibody labeling, is a technique for modification of antibodies which involves with the attachment of a specific tag to an antibody. These labeled antibodies can be used to isolate and purify a protein of interest from a complex mixture, usually cells, tissues or whole organisms.