What are Raji cells used for?
The Raji cell assay is an in vitro method for detecting complement fixing immune complexes in serum samples. The Raji cell is a lymphoblastoid cell with B-cell characteristics derived from a Burkitt’s lymphoma cell line.
Are Raji cells adherent?
Raji is suspension cell line (Growth is in the form of single cells without attachment). Raji A is adherent cell line. To overcome the problem with adherence, RAJI ‘A’ was developed from Raji cell line, which contains typical human chromosomes and the modal total chromosome number (47-48) is the same as that of RAJI.
What are Bjab cells?
The malignant human B-cell-line BJAB is a EBV-negative Burkitt-like lymphoma cell line. Synonyms: BJAB cell. Homo sapiens cell line.
Do Raji cells express CD20?
Raji-Null cells were developed from the human Raji cell line. Raji-Null cells constitutively express different antigens at their cell surface that can be targeted by specific antibodies, notably to induce antibody-dependent cellular cytotoxicity (ADCC). -Human CD20 is highly expressed by Raji cells.
What is HEK293 cell line?
HEK293 is a hypotriploid human cell line. The modal HEK293 chromosome number is 64 in 30% of cells, with higher ploidy occurring in 4.2% of cells. They have three copies of the X chromosome, and a 4 kbp fragment of Ad5 was incorporated into chromosome 19 of the HEK293 genome, which displays cytogenetic instability.
What cells express CD20?
CD20 (cluster of differentiate 20) is a protein that is expressed on the surface of B cells, starting at the pre-B cell stage and also on mature B cells in the bone marrow and in the periphery.
What is HepG2 cell line?
Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. Hep G2 will respond to stimulation with human growth hormone.
What is Nalm6?
Nalm6 is a B cell precursor leukemia cell line initiated from an adolescent male. It is a CD24-positive xenograft model of acute lymphoblastic leukemia.
Are HEK293 immortal?
HEK293 is embryonic kidney immortalized cell line, which represents the epithelial morphology and possibility to form tumors in nude mice. HEK 293 cells are popular for their ease of growth and transfection, making them a common cell culture in cancer research.
What have HEK 293 cells been used for?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.
What is CD in CD20?
CD20 is a CD marker—a molecule on the cell surface that can be used to identify and type a particular cell in the body. CD20 is found on the surface of B cells, but let’s back up and make this easier to understand. Bryn Lennon / GettyImages.
Is CD20 positive bad?
CD20 negative B cell non-Hodgkin lymphoma (NHL) is rare and accounts for approximately 1-2% of B cell lymphomas. CD20- negative NHL is frequently associated with extranodal involvement, atypical morphology, aggressive clinical behaviour, resistance to standard chemotherapy and poor prognosis.
How did the Raji cell line get its name?
Raji cells were derived from the B-lymphocytes of an 11-year-old Nigerian Burkitt’s lymphoma male patient in 1963 by R.J.V. Pulvertaft. The Raji cell line is categorized as lymphoblast-like.
How are Raji cells used as a transfection host?
The Raji cell line is widely used as a transfection host, Raji cells were derived from the B-lymphocytes of an 11-year-old Nigerian Burkitt’s lymphoma male patient in 1963 by R.J.V. Pulvertaft. [citation needed] The Raji cell line is categorized as lymphoblast-like. The culture medium used to grow Raji cells is RPMI supplemented…
What kind of virus does Raji cell line carry?
This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles.
How big is a Raji cell in size?
The cells are relatively large in diameter (5-8 μm), have irregular indented nuclei, and almost extensive cytoplasm with free ribosomes which tend to clump. Raji cells grow as single, non-motile, free-floating (non-adhesion) individuals or doublets to glass. Some cells look elongated, pear-shaped with larger, multinucleate, round cells.