How does the Agilent 2100 Bioanalyzer work?
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA and RNA. The fluorescent dye molecules intercalate into DNA or RNA strands. They are then detected by their fluorescence and translated into gel-like images (bands) and electropherograms (peaks).
How does a Bioanalyzer chip work?
How does the Bioanalyzer work? The bioanalyzer instrument detects biomolecules by laser-induced fluorescence. Each LabChip® contains an interconnected set of microchannels that is used for separation of nucleic acid fragments based on their size as they are driven through it electrophoretically.
What is the Bioanalyzer used for?
The Bioanalyzer can be used to assess the size and quality of DNA over a range of sizes and concentrations. The DNA 1000 assay can be used with extracted, clean DNA to look at the size profile of a sample from 25bp to 1000bp at a concentration of 0.1-50ng/ul.
What is the difference between Bioanalyzer and TapeStation?
A: The Agilent 2200 TapeStation system can run anywhere from 1 to 95 samples. The Bioanalyzer is “chip- based” and can only fit 11 or 12 samples per run. Therefore, the service fee for Bioanalyzer run is charged on a per chip basis. Both Bioanalyzer and TapeStation can run cDNA or NextGen library samples.
How does a Bioanalyzer quantify RNA?
The Bioanalyzer can be used to look at total RNA quality by observing the 18S and 28S ribosomal peaks and their ratio, or 16S and 23S peaks of prokaryotes. The 28S/18S ratio should be 2, but often is much less with the RNA being of good quality. More important than the ratio is the degradation in the peaks.
How do you read Bioanalyzer results?
Even though the ideal ratio is 2.0, the bioanalyzer rarely reflects this ratio. If the ratio is greater than 2.0 it may indicate the presence of sheared single stranded genomic DNA which can run around the 28S band. If the ratio is less than 1.0, there are usually definite degradation problems with the RNA.
What does the Bioanalyzer measure?
The Agilent 2100 Bioanalyzer can be used to measure the quantity, integrity and purity of small RNAs and HMW total ribosomal RNA, and the integration of these two measurements provides many advantages over traditional agarose gel electrophoresis.
How do you run Bioanalyzer?
1 Slowly fill one of the wells of the electrode cleaner with 350 µl deionized analysis-grade water. 2 Open the lid and place the electrode cleaner in the Agilent 2100 Bioanalyzer. 3 Close the lid and leave it closed for about 10 seconds. 4 Open the lid and remove the electrode cleaner.
What is a Agilent Bioanalyzer?
Bioanalyzer Systems The Agilent 2100 Bioanalyzer system is an established automated electrophoresis solution for the sample quality control of biomolecules. The system integrates an instrument, data processing software, reagents, and a microfluidic chip specific for DNA, RNA, or protein analysis.
What is RNA Bioanalyzer?
The Bioanalyzer RNA 6000 Nano assay provides reliable and reproducible characterization of total RNA and mRNA from multiple sample types, from as low as 5ng of total RNA. The Bioanalyzer RNA kits allow dependable and precise integrity checks and sample quantitation prior to any RNA-dependent application.
How does the TapeStation work?
The 2200 TapeStation system is a novel method for determining the concentration, integrity, and molecular weight size of FFPE genomic DNA in one step using only 1 μL of starting material. The system analyzes genomic DNA in the concentration range of 10 ng/μL–100 ng/μL and has a sensitivity of 0.5 ng/μL.
What is ScreenTape?
DNA ScreenTape analysis provides a fast, automated, and flexible DNA electrophoresis solution for sample quality control, e.g in next-generation sequencing workflows. The DNA ScreenTape assays can separate DNA fragments and libraries up to 5000 base pairs in 1-2 minutes/sample.
When was the Agilent 2100 Bioanalyzer system introduced?
Introduced in 1999, the Agilent 2100 Bioanalyzer system was the fi rst commercially available instrument for miniaturized analysis of biological samples with on-chip electrophoresis.
Which is sample quality control system does Agilent use?
Sample quality is critical to experimental success. With citations in more than 50,000 scientific papers, the Agilent 2100 Bioanalyzer system is a well-established tool for quality control of RNA and DNA samples. Applications for DNA, RNA and Protein Analysis.
Is the 2100 Bioanalyzer suitable for GLP / GMP environments?
For GLP/GMP environments, the 2100 Bioanalyzer system offers IQ and OQ services, as well as features to enable 21 CF Part 11 compliance. This animation visualizes electrophoresis on a chip.
Is the 2100 Bioanalyzer compliant with the RoHS Directive?
The 2100 Bioanalyzer instrument is compliant with European RoHS directives. For GLP/GMP environments, the 2100 Bioanalyzer system offers IQ and OQ services, as well as features to enable 21 CF Part 11 compliance. This animation visualizes electrophoresis on a chip.