What is VP16 activation domain?
The VP16 activation domain is one of the most potent transcription activation regions described and is widely used as a model for the action of regulatory proteins and their interactions with the basal transcription machinery.
How does VP16 activate gene expression?
In the early stage of viral infection, VP16 released by invading virions binds to the immediate-early (IE) gene promoter to stimulate the transcription of IE genes as a transactivating factor that acts specifically on IE genes.
What is the function of GAL4 VP16?
The highly acidic C-terminal tail of VP16 functions as a potent transcriptional activator in mammalian cells. GAL4-VP16 construct functions as an activator which promotes transcription in mammalian cells.
Is VP16 a transcription factor?
VP16 is a transcription factor of herpes simplex virus (HSV) type 1 that is involved in the activation of the viral immediate-early genes (Flint and Shenk, 1997; Wysocka and Herr, 2003).
Why is etoposide called VP 16?
Etoposide was first synthesized in 1966 and U.S. Food and Drug Administration approval was granted in 1983. The nickname VP-16 likely comes from a compounding of the last name of one of the chemists who performed early work on the drug (von Wartburg) and podophyllotoxin.
What is transactivation activity?
From Wikipedia, the free encyclopedia. In the context of gene regulation: transactivation is the increased rate of gene expression triggered either by biological processes or by artificial means, through the expression of an intermediate transactivator protein.
What is the purpose of DNA binding domains?
The function of DNA binding is either structural or involves transcription regulation, with the two roles sometimes overlapping. DNA-binding domains with functions involving DNA structure have biological roles in DNA replication, repair, storage, and modification, such as methylation.
What does GAL4 bind to?
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′.
What is GAL4 VP16?
The GAL4::VP16 entry in FlyBase represents an artificial transcriptional activator (‘driver’) that includes the DNA-binding domain from the Saccharomyces cerevisiae GAL4 gene ( SGDID:S000006169 ) and a transcriptional activation domain from the Herpes simplex virus Type 1 VP16 gene (UniProtKB:P06492) (PMID:3047590).
What is the strongest chemotherapy?
Doxorubicin (Adriamycin) is one of the most powerful chemotherapy drugs ever invented. It can kill cancer cells at every point in their life cycle, and it’s used to treat a wide variety of cancers.
What do topoisomerase inhibitors do?
Topoisomerase inhibitors block the ligation step of the cell cycle, which generates DNA single- and double-strand breaks, leading to apoptotic cell death. Topoisomerase I inhibitors include irinotecan, topotecan, and camptothecin, and topoisomerase II inhibitors include etoposide, doxorubicin, and epirubicin.
What are DNA binding Transactivators?
The transactivator gene expresses a transcription factor that binds to specific promoter region of DNA. By binding to the promoter region of a gene, the transcription factor causes that gene to be expressed.