What type of cells are THP-1?
THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.
What are THP-1 monocytes?
THP-1 designates a spontaneously immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 subtype) (3). The use of cultured THP-1 cells in vitro as a model for primary human monocytes ex vivo exemplifies the basic concept of translational research.
What are THP-1 macrophages?
THP-1 cells can be differentiated into macrophage-like cells that resemble properties of mature macrophages by activation of protein kinase C (PKC) with phorbol-12-myristate-13-acetate (PMA), ultimately resulting in cells with increased adherence and loss of proliferative activity (Schwende et al., 1996).
Are THP-1 cells adherent or suspension?
Normally THP-1 cells grow in suspension, but they can be induced to differentiate into adherent macrophage-like cells, which express IL-8 and other markers, and can thus serve as a model system for differentiation.
How do you thaw THP 1 cells?
To Successfully Freeze THP-1 Cells, Follow These Tips: Thaw frozen vials in a 37°C water bath, but DO NOT LET THE WATER COME IN CONTACT WITH THE LID. Thaw the contents until there’s a small lump of ice in the bottom—trust me, it may seem counter-intuitive, but it works.
What is Vero cell culture?
Vero cells are a lineage of cells used in cell cultures. The ‘Vero’ lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species).
What is meant by monocyte?
A monocyte is a type of white blood cell and a type of phagocyte. Enlarge. Blood cells. Blood contains many types of cells: white blood cells (monocytes, lymphocytes, neutrophils, eosinophils, basophils, and macrophages), red blood cells (erythrocytes), and platelets.
How do you revive THP-1 cells?
To revive THP-1 and Jurkat cells from frozen liquid N2 stocks, warm RPMI-1640 supplemented with FBS (10%, complete media) to 37 °C in a water bath. 2. Thaw the capped frozen vial containing cells in a water bath at 37 °C. Note: Only a partial thaw is sufficient, and a small lump of ice should remain in the vial.
How big is a Vero cell?
17 micrometers
Seems to indicate (slide 15) an approximate diameter of 17 micrometers.
How is Rage inhibited in THP-1 monocytes?
In the present study, RAGE in THP-1 monocytes was inhibited by shRNA lentiviral particles, followed by exposure to IH. Cell adhesion assay, transwell migration assay, and macrophage polarization assays were performed to study the effects of IH and RAGE.
How does IH affect THP-1 monocyte adhesion?
Cell adhesion assay, transwell migration assay, and macrophage polarization assays were performed to study the effects of IH and RAGE. The mRNA and protein expression levels were investigated by RT/real-time PCR and western blot analysis, respectively. We found that IH increased RAGE expression and activated NF-кB signalling in THP-1 monocytes.
Is the THP-1 cell line suitable as a transfection host?
If you use alternative products, the quality and effectiveness of the item may be affected. These cells are suitable as a transfection host. Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
Can a THP-1 cell spontaneously phagocytose a bacteria?
THP-1 cells have been shown to spontaneously phagocytose bacteria without the need for activation or differentiation 20.