What is mcf7 cell line?
MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].
Is MCF10A triple negative?
EGFR activating mutations mediate EGF-independent proliferation in MCF10A. As triple negative breast cancers (TNBC) have been found to harbour mutations in EGFR [28], we wished to determine how they affect a relatively normal breast cell line.
What is a cell line in cell culture?
Cell line is a general term that applies to a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. Cell lines are usually clonal, meaning that the entire population originated from a single common ancestor cell.
Is MCF7 primary cell line?
It is ER-positive and progesterone receptor (PR)-positive (8) and belongs to the luminal A molecular subtype (2). MCF-7 is a poorly-aggressive and non-invasive cell line (9), normally being considered to have low metastatic potential (8).
Does MCF7 express HER2?
The MCF7 breast cancer cell line was transfected with a pcDNA 3.1 construct encoding human HER2 that was derived from a retroviral construct (Riese et al, 1995), and HER2 expressing clones were identified by immunoblot analysis (Figure 1A).
What are BT 20 cells?
BT-20 is a breast cancer cell line derived from a 74-year-old human female in 1958 by E.Y. Lasfargues and L. Ozzello. The cells technically came from a triple-negative breast cancer, which was caused by an invasive ductal carcinoma in the mammary gland.
What is TNBC cell line?
The latter group is referred to as triple-negative breast cancer (TNBC) based on the lack of these three molecular markers. Generally, hormone receptor expressing breast cancers have a more favorable prognosis than either those with HER-2/Neu amplification or those that are triple-negative [5].
What is in cell culture media?
Cell culture media generally comprise an appropriate source of energy and compounds which regulate the cell cycle. A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, hormones, and attachment factors.
Are cell culture and cell line same?
Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. After the first subculture, the primary culture becomes known as a cell line or subclone.
What type of cells is MCF7?
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers.
Are MCF7 cells metastatic?
All Answers (7) No, MCF-7 is considered metastatic. The non-tumorous and non-metastatic line is MCF-10A or MCF-12A.
Where did the MCF10A cell line come from?
A human cell line (MCF10A) originated from spontaneous immortalization of breast epithelial cells obtained from a patient with fibrocystic disease. MCF10A cells do not survive in vivo in immunodeficient mice. However, T24 c-Ha-ras oncogene-transfected MCF10A cells (MCF10AT) form small nodules in nud …
Can a MCF10A cell survive in vivo?
MCF10A cells do not survive in vivo in immunodeficient mice. However, T24 c-Ha-ras oncogene-transfected MCF10A cells (MCF10AT) form small nodules in nude/beige mice that persist for at least 1 year and sporadically progress to carcinomas.
How are MCF10A cells expressed in two dimensional culture?
MCF10A cells expressed markers representing luminal, basal, and progenitor phenotypes in two-dimensional (2D) culture. When grown in suspension culture, MCF10A cells showed low mammosphere-forming ability. Cells in mammospheres and 3D culture expressed both luminal and basal markers.
What kind of supernatant to use for MCF10A cells?
After 48 h viral supernatant was harvested, filtered (45 μm) and used to infect MCF10A cells in the presence of 8 μg/mL polybrene (Sigma-Aldrich).