What is the purpose of macromolecules lab?
PURPOSE: To use indicators to test for the presence of lipids, carbohydrates, and proteins in various foods. HYPOTHESIS: Which foods will contain which macromolecules?
Which macromolecules will you be testing for in lab?
You will be testing mainly for the presence of simple carbohydrates (glucose, sucrose), complex carbohydrates (starch), and proteins. Below are the indicators you will use to detect for these macromolecules. Benedict’s reagent changes color when exposed to a reducing agent, and all monosaccharides are reducing agents.
What is the purpose of testing distilled water for macromolecules?
Distilled water is basically inert, meaning nothing is in the water but hydrogen and oxygen. Distillation kills most organic matter and removes minerals from the water, making it an ideal control element for science projects and laboratory tests.
What do you know about macromolecules?
A macromolecule is a very large molecule, such as a protein. They are composed of thousands of covalently bonded atoms. The most common macromolecules in biochemistry are biopolymers (nucleic acids, proteins, and carbohydrates) and large non-polymeric molecules such as lipids and macrocycles.
What do you use to test the macromolecule monosaccharide?
Test #1: Testing for Sugars [Monosaccharides & Disaccharides] To test for the presence of monosaccharides and disaccharides, we will use the Benedict’s test for reducing sugars.
How does an indicator detect the presence of a macromolecule?
We can use indicator solutions to test for the presence of our macromolecules of life! We say that an indicator test is positive when the color change indicates that the macromolecules is present and negative if there is no color change indicating the lack of the macromolecule.
What test do we use to test for proteins?
Biuret test
The presence of protein is tested by the Biuret test for proteins. The Biurette reagent made of sodium hydroxide and copper (II) sulphate helps in determining the presence of protein in a sample.
What conclusion could you make if a positive test for any of the macromolecules occurred in the test tube containing only distilled water?
Answer: If a positive test for any macromolecules occurred in the test tube containing only distilled water. There might be a chemical added and may not be visible as one of the macromolecules that shows up when adding the chemical indicator.
What is the composition of macromolecules?
Macromolecules are made up of basic molecular units. They include the proteins (polymers of amino acids), nucleic acids (polymers of nucleotides), carbohydrates (polymers of sugars) and lipids (with a variety of modular constituents).
What are the four major categories of macromolecules describe the basic function of each?
Nucleic acids: Stores and transfers info. Carbohydrates; Store energy, provide fuel, and build structure in body, main source of energy, structure of plant cell wall. Lipid: Insulator and stores fat and energy. Protein: Provide structural support,transport, enzymes, movement, defense.
When testing for monosaccharides what indicator will you use?
Benedict’s reagent is the indicator we use to detect monosaccharides. When monosaccharides are mixed with Benedict’s and heated, a color change occurs. If there is a small amount of monosaccharide in the solutions, a greenish solution is produced.
How are macromolecules tested in the life lab?
This lab was done to test for macromolecules consisting of carbohydrates, lipids, proteins, and nucleic acids by using specific reagents to test for each. The result would bring out a specific color change in the macromolecule.
How is the third lab for carbohydrates created?
The third lab for carbohydrates consisted of cleaning four test tubes and labeling them 1 through 4. Each test tube was filled with 10 drops respectively of distilled water, glucose solution, sucrose solution, and starch solution. Three drops of iodine were added to each tube and swirled.
What are bio 113 biological molecules of life lab report?
Bio 113 Biological Molecules of Life Lab Report. Proteins and peptides react with the Biuret reagent. This reaction is specific for compounds with more than two peptide bonds. Biuret reagent is a mixture of a strong solution of sodium or potassium hydroxide and small amount of very dilute copper sulfate.