What tests are performed in a microbiology lab?
List of Laboratory Tests – Microbiology
- Aspirate culture & sensitivity.
- Aspirate for AFB.
- Blood culture & sensitivity.
- Cholera ag.
- Chlamydia.
- CSF- culture & sensitivity.
- Ear culture & sensitivity.
- Endocervical swab.
What are microbiology lab techniques?
Microbiologists use five basic procedures to examine and characterize microbes: Inoculation, Incubation, Isolation, Inspection (observation), and Identification—the five “I’s.” To culture a microorganism a small sample, the inoculum, is introduced into a culture medium usually with a platinum wire probe streaked across …
What is microbiology food testing?
Microbiological testing of food is the examination of the microscopic organisms in food. These organisms could be single cell, multiple cell or without cell. Microbiology includes various sub-disciplines like Virology, Mycology, Parasitology and Bacteriology.
What are microbiological test how it can performed?
Microbiological analysis covers the use of biological, biochemical or chemical methods for the detection, identification or enumeration of microorganisms. It is often applied to disease causing and spoilage microorganisms. Impact of water. Impurities. Products.
What are hematology lab tests?
Hematology tests include tests on the blood, blood proteins and blood-producing organs. These tests can evaluate a variety of blood conditions including infection, anemia, inflammation, hemophilia, blood-clotting disorders, leukemia and the body’s response to chemotherapy treatments.
What are the requirements in a microbiology laboratory?
A modern microbiology laboratory should be furnished with the following equipment.
- Hot Air Oven for Sterilization:
- Drying Oven:
- Autoclave:
- Microbiological Incubator:
- BOD Incubator (Low Temperature Incubator):
- Fridge (Refrigerator):
- Deep-fridge:
- Electronic Top-pan Balance:
What five basic techniques are used to identify a microorganism in the laboratory?
The Five I’s is a method used to locate, grow, observe and characterize microorganisms which include inoculation, incubation, isolation, inspection and identification.
Which method is used for food testing?
For chemical testing, different analytical techniques are commonly used. These include traditional, so-called ‘wet chemical’ techniques, and advanced approaches such as infrared spectroscopy, gas and liquid chromatography and mass spectrometry.
How do you test for bacteria in food?
The first step in the new test for bacteria detection is to collect a sample of water, juice or mashed vegetable leaf and place the chemical-based detection chip in with the sample. The standard method for culturing bacteria from food samples, known as an aerobic plate count (APC) takes two days, He explained.
What is a metabolic test in microbiology?
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. The ability of bacteria to form organic compounds by metabolizing certain carbohydrates and related compounds is a widely used method for the …
What other method may be used for indole testing?
Additional biochemical testing using pure cultures is recommended for complete identification. The tube test is a more sensitive method of detecting indole than the spot test. When performing a spot test, Kovacs Indole Reagent may be used as a substitute for the spot test reagent.
How is the microbiological examination of food done?
When examining foods, the possibility of detecting the presence of micro-organisms by looking at a sample directly under the microscope should not be missed. A small amount of material can be mounted and teased out in a drop of water on a slide, covered with a cover slip, and examined, first with a low magnification, and then with a x 45 objective.
When to take a food sample to the lab?
Samples of foods will be available in the laboratory up to a week before your scheduled lab period. Transfer 5 ml of Tryptic Soy Broth to a disposable plastic culture tube. You should select one food sample for testing.
Are there chemicals used in the microbiology laboratory?
In addition to microorganisms, there are some chemicals used in this laboratory that are potentially harmful. Many procedures involve glassware, open flames, and sharp objects that can cause trauma/ damage if used improperly.
What are the rules for working in a laboratory?
The laboratory procedures must be read prior to attending the laboratory session. Smoking, eating, and drinking in the laboratory are absolutely prohibited in the laboratory at any time. 2. Only closed-toe shoes are to be worn in the laboratory.