How do you measure antioxidant levels?
ORAC Assay (The Oxygen Radical Absorbance Capacity) ORAC assay is a method for quantifying the antioxidant strength of substances. It involves combining the sample to be tested (i.e. the antioxidant) with a fluorescent compound as well as a compound that generates free radicals at a known rate.
What is TPC in honey?
Total phenolic content (TPC) There is a positive correlation coefficient between total phenolic, DPPH, FRAP and β-Carotene methods indicating that these compounds are mainly responsible for the antioxidant capacity of honey. Chua et al.
Is honey powerful in antioxidant?
Honey Sweetens Your Health. Researchers say honey contains varying concentrations of polyphenols, which are powerful antioxidants that are thought to reduce the risk of heart disease and cancer. Polyphenols are also found in fruits, vegetables, tea, and olive oil.
What is the unit of antioxidant?
ORAC: Measuring unit of the strength of an Antioxidant The answer is Oxygen Radical Absorbance Capacity (ORAC) of an Antioxidant. The power and strength of an Antioxidant product is measured with a value which is known as ORAC.
How do you calculate antioxidant properties?
The capability to scavenge the DPPH radical was calculated using the following equation [4]. DPPH Scavenged (%)= ((AB–AA)/AB)×100….. (1), where, AB is absorbance of blank at t= 0 min; AA is absorbance of the antioxidant at t= 30 min.
What is total antioxidant activity?
Total antioxidant capacity (TAC) is the measure of the amount of free radicals scavenged by a test solution [4], being used to evaluate the antioxidant capacity of biological samples [3, 6, 7].
Which honey has the most antioxidants?
Buckwheat honey
Buckwheat honey actually has one of the highest antioxidant counts of any honey on the planet!
How many antioxidants are in honey?
Among biomarkers phenolic compounds, the main antioxidants reported in honey have been intensively examined. More than 150 phenolic compounds in honey have been investigated, including phenolic acids, and flavonoids [6,18].
How do you calculate total antioxidant capacity?
Total antioxidant capacity (Ta) was calculated with the equation Ta = 100/(OD1 + 2 * OD2 + 2 * OD3 + 2 * OD4 + OD5), and average activity (A) was calculated as A = Ta/M. Results.
How is ORAC measured?
It’s a lab test that attempts to quantify the “total antioxidant capacity” (TAC) of a food by placing a sample of the food in a test tube, along with certain molecules that generate free radical activity and certain other molecules that are vulnerable to oxidation .
How do you calculate the IC50 value of antioxidant activity?
you can calculate the IC50 as follows: IC50 = (0.5 – b)/a. You replace (y) by 50 in the equation y = 1.3534 x + 0.58 and you deduce ( x) .
How is the antioxidant capacity of honey related?
The antioxidant capacity of the honey samples was correlated with their biochemical constituents such as total phenol, total flavonoid content, and total water-soluble vitamins (vitamin B1, B2, B3, B9, B12, and vitamin C).
What is the phenolic content of fir honey?
Phenolic content expressed as gallic acid equivalent ranged from 44.8 mg/kg in acacia honey to 241.4 mg/kg in fir honey. Antioxidant activity was the lowest in the brightest acacia and lime honeys and the highest in darker honeys, namely fir, spruce and forest.
Are there any water soluble vitamins in honey?
Water-Soluble Vitamins in Honey. In addition to polyphenols, the antioxidant activity of honey might be also attributed to the presence of several water-soluble vitamins such as vitamin B1 (thiamine), B2 (riboflavin), B3 (nicotinic acid), B9 (folic acid), B12 (cyanocobalamin), and vitamin C as presented in Table 2.