What does high inoculum mean?
The inoculum effect (IE) is a laboratory phenomenon that is described as a significant increase in the minimal inhibitory concentration of an antibiotic when the number of organisms inoculated is increased. The IE generally occurs with beta-lactam antibiotics in relation to beta-lactamase-producing bacteria.
What is inoculum in microbiology?
inoculum A small amount of material containing bacteria, viruses, or other microorganisms that is used to start a culture.
How do you calculate inoculum volume?
If you intent is to establish by inoculation 50 liters at 10^5/ml, think you’ll need 500 ml of 10^7/ml inoculum in those 50 liters (i.e. 500ml inoculum in 49.5 liters). Consider: concentration x volume = concentration x volume.
What is inoculum density?
The inoculum density (ID) in a sample was estimated as number of microsclerotia per gram of dry soil, and it was recorded for each sample area studied. Symptom severity. Severity of plant symptoms was assessed on a 0-to-100% rating scale adapted from Cirulli et al.
What is the normal range of inoculum size used in fermentation?
105 to 107
(2013), respectively. Most commonly an inoculum size ranging from 105 to 107 spores/g of substrate is used (Silva et al., 2005; Botella et al., 2005; Hendges et al., 2011, Demir and Tari, 2016).
What is inoculum with example?
The definition of an inoculum is a substance introduced into the body to create or increase the body’s resistance or immunity to a disease. A flu vaccine is an example of an inoculum. The active material used in an inoculation; an inoculant.
What is the standard inoculum size of fermentation process?
Most commonly an inoculum size ranging from 105 to 107 spores/g of substrate is used (Silva et al., 2005; Botella et al., 2005; Hendges et al., 2011, Demir and Tari, 2016).
What is inoculum in pathology?
Inoculation is the pathogen or any part of the pathogen that contacts with the plant at certain site to initiate the infection process, such as spores, sclerotia, or fragments of mycelium of fungi may be fungal inoculum.
What do you mean by inoculum?
Definition. noun, plural. (1) Cells used in an inoculation, such as cells added to start a culture. (2) A biological material (like a virus or toxin or immune serum) that is injected into a human to induce or increase immunity to a particular disease.
How do you make an inoculum?
6.1. Inocula are prepared by growing C. neoformans in liquid YPAD overnight at 30 °C. Cells are counted by hemocytometer and, for an intranasal infection, 1×107 cells are washed twice with PBS and resuspended in 1 ml of PBS. Fifty microliters of this inoculum are used per mouse (5×105 cells).
What is the importance of inoculum?
The inoculum source had a significant impact on both growth and pH tolerance of the bacterial community in the EL soil. These differences between the EL soil inoculated with UL soil and FL soil were correlated to structural changes, as evidenced by both PLFA and DGGE analyses on the soil.
What is the standard inoculums size of fermentation process?
Which is the correct definition of inoculum size?
inoculum size is the required concentration of expected microorganism for a standard test. it is a believe to expect the standard suspension prepare is the inoculum size for a particular yield at different dilution rate for either pilot or industrial scale analysis.
What do you call material used in inoculum?
The material used in an inoculation. Also called inoculant. [New Latin, from Latin inoculāre, to graft a scion; see inoculate.] American Heritage® Dictionary of the English Language, Fifth Edition.
Which is the best definition of an inocula?
inoculum- a substance (a virus or toxin or immune serum) that is introduced into the body to produce or increase immunity to a particular disease
Which is the best definition of an inoculant substance?
1. inoculum- a substance (a virus or toxin or immune serum) that is introduced into the body to produce or increase immunity to a particular disease inoculant substance- a particular kind or species of matter with uniform properties; “shigella is one of the most toxic substances known to man”