What is phosphotungstic acid method?
A rapid, convenient one-tube method for serum uric acid is described which employs a single addition of phosphotungstic acid. Serum proteins are precipitated and sufficient phospho-18-tungstic acid remains in the supernatant solution for oxidation of uric acid following alkalinization with sodium carbonate.
What is the purpose of phosphotungstic acid?
Phosphotungstic acid is used in histology as a component for staining of cell specimens, often together with haematoxylin as PTAH. It binds to fibrin, collagen, and fibres of connective tissues, and replaces the anions of dyes from these materials, selectively decoloring them.
How to prepare phosphotungstic acid?
A method for the production of phosphotungstic acid which comprises preparing an aqueous solution of sodium tungstate, heating the solution to a temperature between about 70′ and C., maintaining the solution at said temperature and slowly adding hydrochloric acid thereto until the solution reaches a pH value of from …
Is phosphotungstic acid light sensitive?
Chemical Properties Light Sensitive. Store under Nitrogen. Ambient temperatures. Soluble in water, dichloromethane, acetonitrile, acetic acid and ionic liquids.
Can Phosphotungstic precipitate proteins?
Many proteins derived from the latex of Hevea brasiliensis that remain soluble in trichloroacetic acid (TCA) can be precipitated by phosphotungstic acid (PTA). A combination of 5% TCA and 0.2% PTA precipitates a wide range of proteins effectively even when they are present in low concentrations (below 1 μg ml−1).
What is the role of phosphomolybdic acid?
Phosphomolybdic is used as a stain for developing thin-layer chromatography plates, staining phenolics, hydrocarbon waxes, alkaloids, and steroids. Conjugated unsaturated compounds reduce PMA to molybdenum blue. Phosphomolybdic acid is also occasionally used in acid-catalyzed reactions in organic synthesis.
How is uric acid reagent prepared?
Uric Acid reagent: 4-AAP >0.2mM, HDCBS 2mM, Uricase (Microbial) >150 U/L, Peroxidase (horseradish) >2,500 U/L, Buffer, pH 8.1 ± 0.1, Non-reactive stabilizers. The reagent is ready to use. The reagent set is stored at 2-8°C. Under proper storage the reagent will remain stable until the indicated expiration date.
How does acetonitrile precipitate proteins?
4.1 Protein Precipitation PPT involves the addition of an organic solvent, such as methanol or acetonitrile to a patient sample. The organic solvent causes the proteins in the patient sample to precipitate out of solution, and after centrifugation, the proteins form a pellet at the bottom of the tube.
Is tannic acid can precipitate proteins?
Tannins (or tannic acid) are water-soluble phenolic compounds with a molecular weight greater than 500 and with the ability to precipitate proteins from aqueous solution.
What is the formula of ammonium Phosphomolybdate?
(NH4)3PMo12O40
Ammonium phosphomolybdate/Formula
How do you make a phosphomolybdic acid reagent?
A3.1 GENERAL STAINS Phosphomolybdic Acid (PMA): Dissolve 10 g of phosphomolybdic acid in 100 mL of absolute ethanol. Potassium Permanganate: Dissolve 1.5 g of KMnO4, 10 g of K2CO3, and 1.25 mL of 10% NaOH in 200 mL of water. Vanillin: Dissolve 15 g of vanillin in 250 mL of ethanol and add 2.5 mL of conc. sulfuric acid.
What is the formula for phosphotungstic acid hydrate?
Synonym (s): 12-Phosphotungstic acid hydrate Formula: H3PO4 12WO3 xH2O Formula weight: 2880.20 (anhy) CAS Number: 12501-23-4 UN Number: UN3260 Hazard Class: 8 Packing Group: III Harmonized Tariff Code: 2811.19 Hazard Statements: H314-H318 Causes severe skin burns and eye…
Why is phosphotungstic acid used as a staining agent?
Phosphotungstic acid hydrate may be used as a staining agent for the transmission electron microscope (TEM) imaging of cetyltrimethylammonium bromide (CTAB) coated gold nanorods. Phosphotungstic acid doped ormosil hybrid networks.
Which is the stable phosphotungstic acid Am IV?
Americium (IV) is stable in concentrated H 3 PO 4, K 4 P 2 O 7, phosphotungstate, and fluoride (NH 4 F, KF) solutions, and is otherwise reduced to Am III.
What is the melting point of phosphotungstic acid?
EPTA is the name of ethanolic phosphotungstic acid, its alcohol solution used in biology. It has the appearance of small, colorless-grayish or slightly yellow-green crystals, with melting point 89 °C (24 H 2O hydrate). It is odorless and soluble in water (200 g/100 ml).