What does a Nucleofector do?
A Nucleofector® Device that comprises unique electrical parameters pre-programmed for each optimized cell type, to deliver the substrate directly into the cell nucleus and the cytoplasm.
What is Amaxa?
The Amaxa Nucleofector technology is a transfection system designed to transfer genes into cells quickly and efficiently. The technology is based on electroporation. Each cell type is introduced to the DNA (or RNA) in an optimized Nucleofector solution that has been designed for that particular cell type.
What is an electroporation machine?
Electroporation is a physical transfection method that uses an electrical pulse to create temporary pores in cell membranes through which substances like nucleic acids can pass into cells.
What is 4D Nucleofector?
The 4D-Nucleofector® X Unit is one of the four functional modules of the 4D-Nucleofector® System. It supports Nucleofection of various cell numbers (2 x 104 to 2 x 107) cells in different formats. There are cell type-specific Optimized Protocols or recommendations available in our knowledge database.
What is flow electroporation?
Flow electroporation (EP) permits cells to be electroporated while they flow between two electrodes and a transient electrical field is experienced by the cells as they pass between these electrodes.
What cell type is used in electroporation?
It is non-viral, non-toxic and can be used on all cell types including mammalian, bacteria, algae, plant and yeast. It can be used on cells in all forms, in vitro or in vivo/ex vivo. In vitro is Latin for “within glass” and includes suspension cell, tissue slice/whole organ, and adherent cell.
How is electroporation done?
Electroporation is based on a simple process. Host cells and selected molecules are suspended in a conductive solution, and an electrical circuit is closed around the mixture. An electrical pulse at an optimized voltage and only lasting a few microseconds to a millisecond is discharged through the cell suspension.
What current does electroporation use?
Electroporation involves application of high voltage (>50 V) to create transient pores in the stratum corneum, leading to a large increase in drug transport, delivery of macromolecules, a quick drug effect onset, and insignificant or only minor skin damage (Golden et al., 1987; Regnier and Preat, 1998; Hofmann, 1999).
What is Biolistics cell type?
Biolistics is another method used for gene insertion, whereby nano-sized particles of heavy metals (e.g., gold) are coated with DNA and injected into cells with a “gene gun.”
How is the 4D Nucleofector used in electroporation?
However, the 4D-Nucleofector TM Y Unit offers the opportunity to keep cells in adherence during electroporation. Adherent primary cells, especially neurons, at defined developmental stages can be transfected using the Y Unit without affecting their functionality and with an efficiency of up to 70%.
How is the Ingenio electroporation kit used in science?
Ingenio® Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. See the recommended experimental conditions (PDF). Ingenio® Outperforms Other Electroporation Solutions in Efficiency and Viability.
How is electroporation used in DNA transfection?
Electroporation is a physical transfection method that permeabilizes the cell membrane by applying an electrical pulse and moves molecules via the electrical field into the cell. It is a powerful tool for transfecting large DNA fragments and achieving good transfection efficiencies in cell lines.
When did nucleofector technology come to the market?
Our solution is an improved electroporation technology, the Nucleofector TM Technology, originally introduced into the market by legacy Amaxa in 2001.